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> AMY 124 ELISA Kit (Sheep)

AMY 124 ELISA Kit (Sheep)

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Référence OKCD00126

Conditionnement : 96Wells

Marque : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for AMY 124 ELISA Kit (Sheep) (OKCD00126)
Product Info
Predicted Species ReactivityOvis aries|Sheep
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)89-103%90-98%93-101%96-104%
EDTA plasma(n=5)83-97%86-95%89-99%95-103%
heparin plasma(n=5)81-104%97-105%92-103%99-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Alpha (IFNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Alpha (IFNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interferon Alpha (IFNa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.62-1000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)93-10297
EDTA plasma(n=5)94-10298
heparin plasma(n=5)78-9981
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Amy 124 Microplate96 Wells (12 x 8 Well Strips)
Amy 124 Lyophilized Standard2 x 1 ng
100X Biotinylated Amy 124 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity< 5.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interferon Alpha (IFNa). No significant cross-reactivity or interference between Interferon Alpha (IFNa) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolAMY 124
Gene Full Nameolfactomedin 1
Alias SymbolsAMY, Noe1, OlfA, AW742568, Pancortin, Pancortin3
NCBI Gene Id56177
Protein NameInterferon alpha
Description of TargetInterferon alpha
Uniprot IDQ28561
Protein Accession #NP_001033701
Nucleotide Accession #NM_001038614.1
Protein Size (# AA)195