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> Anti-Chicken IgG (H&L) (Fluorescein Conjugated) Pre-Adsorbed Secondary Antibody

Anti-Chicken IgG (H&L) (Fluorescein Conjugated) Pre-Adsorbed Secondary Antibody

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Référence ASR2654

Conditionnement : 1mg

Marque : Abcepta

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  • WB - Anti-Chicken IgG (H&L) (Fluorescein Conjugated) Pre-Adsorbed Secondary Antibody ASR2654
    Western Blot of Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule . Lane 2: Chicken IgG F(c) Fragment . Lane 3: Chicken IgG F(ab) Fragment . Lane 4: Chicken IgM Whole Molecule . Lane 5: Chicken Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 72 kDa for Chicken IgG and Serum, 25 kDa for F(c) and F(ab), 75 kDa for IgM. Chicken F(c) migrates slightly higher.
Product Information
Description Anti-CHICKEN IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins)
Host Goat
Conjugate Fluorescein (FITC)
FP Value 2.8 moles Fluorescein (FITC) per mole of IgG
Target Species Chicken
Reactivity Chicken
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, FC
Application Note FLISA 1:10,000-1:50,000
IF Microscopy 1:1,000-1:5,000
FlowCytometry 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Chicken IgG whole molecule
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum.   No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.