Anti-Flavocytochrome b558 (Human) mAb (Monoclonal Antibody)
| Target | Flavocytochrome b |
|---|---|
| Product Type | Antibody |
| Application | FCM |
| Clonality | Monoclonal |
| Conjugate | Unlabeled |
| Isotype | IgG1 |
| Immunogen | Human cytochrome b rich fraction |
| Host Species | Mouse |
| Species Reactivity | Human |
| Formulation | 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Research Area | Cell Biology |
| Description/Background | The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2-), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome b558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2-. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections. |
| Storage Temperature | This antibody solution is stable for one year from the date of purchase when stored at -20°C. |
| Protocols | FCM |
| Clone Number | 7D5 |
| Concentration | 1 mg/mL |
| Source | This antibody was purified from hybridoma (clone 7D5) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0 with Balb/c mouse splenocyte immunized with the human cytochrome b rich fraction. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
D162-3
Antibody
100 µl