Anti-MYOC antibody (25-46) {FITC}

Clonality:	Polyclonal
Conjugation:	FITC
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.75 µg/µl
Dilution Range:	WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200 IHC: 1:200 ICC: 1:200 IF: 1:200 CFM: 1:200
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	MYOC
Gene ID:	4653
Uniprot ID:	MYOC_HUMAN
Immunogen Region:	25-46
Immunogen:	Synthetic peptide corresponding to N terminal amino acids 25-46 of Myocilin. Peptide was covalently modified and conjugated to KLH.

Tissue Specificity	Detected in aqueous humor. Detected in the eye (at protein level). Widely expressed. Highly expressed in various types of muscle, ciliary body, papillary sphincter, skeletal muscle, heart, and bone marrow-derived mesenchymal stem cells. Expressed predominantly in the retina. In normal eyes, found in the inner uveal meshwork region and the anterior portion of the meshwork. In contrast, in many glaucomatous eyes, it is found in more regions of the meshwork and seems to be expressed at higher levels than in normal eyes, regardless of the type or clinical severity of glaucoma. The myocilin 35 kDa fragment is detected in aqueous humor and to a lesser extent in iris and ciliary body.
Post Translational Modifications	Different isoforms may arise by post-translational modifications. Glycosylated. Palmitoylated. Undergoes a calcium-dependent proteolytic cleavage at Arg-226 by CAPN2 in the endoplasmic reticulum. The result is the production of two fragments, one of 35 kDa containing the C-terminal olfactomedin-like domain, and another of 20 kDa containing the N-terminal leucine zipper-like domain.
Function	Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling. Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling. Mediates myelination in the peripheral nervous system through ERBB2/ERBB3 signaling. Plays a role as a regulator of muscle hypertrophy through the components of dystrophin-associated protein complex. Involved in positive regulation of mitochondrial depolarization. Plays a role in neurite outgrowth. May participate in the obstruction of fluid outflow in the trabecular meshwork.
Protein Name	Myocilin Myocilin 55 Kda Subunit Trabecular Meshwork-Induced Glucocorticoid Response Protein Cleaved Into - Myocilin - N-Terminal Fragment Myocilin 20 Kda N-Terminal Fragment - Myocilin - C-Terminal Fragment Myocilin 35 Kda N-Terminal Fragment
Cellular Localisation	Secreted Golgi Apparatus Cytoplasmic Vesicle Extracellular Space Extracellular Matrix Extracellular Exosome Mitochondrion Mitochondrion Intermembrane Space Mitochondrion Inner Membrane Mitochondrion Outer Membrane Rough Endoplasmic Reticulum Cell Projection Cilium Located Preferentially In The Ciliary Rootlet And Basal Body Of The Connecting Cilium Of Photoreceptor Cells And In The Rough Endoplasmic Reticulum It Is Only Imported To Mitochondria In The Trabecular Meshwork Localizes To The Golgi Apparatus In Schlemm's Canal Endothelial Cells Appears In The Extracellular Space Of Trabecular Meshwork Cells By An Unconventional Mechanism Likely Associated With Exosome-Like Vesicles Localizes In Trabecular Meshwork Extracellular Matrix Myocilin C-Terminal Fragment: Secreted N-Terminal Fragment: Endoplasmic Reticulum Remains Retained In The Endoplasmic Reticulum
Alternative Antibody Names	Anti-Myocilin antibody Anti-Myocilin 55 Kda Subunit antibody Anti-Trabecular Meshwork-Induced Glucocorticoid Response Protein Cleaved Into - Myocilin - N-Terminal Fragment antibody Anti-Myocilin 20 Kda N-Terminal Fragment - Myocilin - C-Terminal Fragment antibody Anti-Myocilin 35 Kda N-Terminal Fragment antibody Anti-MYOC antibody Anti-GLC1A antibody Anti-TIGR antibody

Information sourced from Uniprot.org

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