Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated
Référence 611-1302
Conditionnement : 2mg
Marque : Rockland Immunochemicals
Specifications for Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated
Product Details
Goat Anti-Rabbit IgG (H&L) Antibody Peroxidase Conjugated - 611-1302
Goat anti-Rabbit IgG Secondary Antibody Peroxidase Conjugated, Goat anti-Rabbit IgG Secondary Antibody HRP Conjugated, GAR-HRP, Anti-rabbit secondary antibody, anti-rabbit HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-rabbit horseradish peroxidase conjugated secondary antibody
Goat
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Rabbit
Rabbit IgG whole molecule
RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Application Details
ELISA, WB
IHC - View References
Anti-Rabbit IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Formulation
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
Anti-Rabbit IgG Antibody peroxidase generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to peroxidase.
References (110)
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E, EIA; IHC, ICC, Histology; WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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IHC, ICC, Histology PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA; IHC, ICC, Histology PubMed
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WB, IB, PCA Source
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Applications
EIA, ELISA PubMed
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Applications
E, EIA; WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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Other PubMed
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