Anti-Rat IgG IgA IgM (H&L) (Biotin Conjugated) Secondary Antibody

Référence ASR2824

Conditionnement : 2mg

Marque : Abcepta

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Product Information
Description Anti-Rat IgG IgA IgM (H&L) (GOAT) Antibody Biotin Conjugated
Host Goat
Conjugate Biotin
Target Species Rat
Reactivity Rat
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:150,000
Western Blot 1:2,000-1:20,000
Immunochemistry 1:1,000-1:10,000
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG IgA IgM
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Rat IgG IgA IgM (H&L) was produced by repeated immunization with Rat IgG, IgA and IgM whole molecules in goat.
Reconstitution Volume 2.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgA, Rat IgG and Rat IgM.  This reagent is suitable for the detection of all rat immunoglobulin subclasses, isotypes and chain combinations.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
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Background

Anti-Rat IgG IgA IgM (H&L) biotin conjugated antibody generated in goat detects specifically rat IgG IgA IgM (H&L). This secondary biotin conjugated antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Availability: 2-3 weeks
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Cat# ASR2824