Application
| WB, ICC |
---|---|
Primary Accession | Q96DT6 |
Other Accession | NP_116241.2 |
Host | Rabbit |
Reactivity | Human, Mouse |
Clonality | Polyclonal |
Description | Rabbit Anti-Human ATG4C Polyclonal |
Target/Specificity | Predicted molecular weight at ~52.5kDa. Observed molecular weights at ~45-60kDa. |
Other Names | APG4 autophagy 4 homolog C Antibody, APG4 C Antibody, Atg4c Antibody, AUTL1 Antibody, Autophagin 3 Antibody, Cysteine protease ATG4C Antibody |
Immunogen | Synthetic peptide from the N-terminal of human ATG4C |
Purification | Peptide Affinity Purified |
Storage | -20ºC |
Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | A 1:1000 dilution of SPC-632 was sufficient for detection of ATG4C on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody. |
Cellular Localization | Cytoplasm |
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Provided below are standard protocols that you may find useful for product applications.
Background
ATG4C belongs to the peptidase C54 family. It is a cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. It also has an activity of delipidating enzyme for the PE-conjugated forms (1).
References
1. Li M., et al. (2011) J Biol Chem. 286: 7327-7338.