beta Tubulin antibody [D66]
Référence GTX11307-100ul
Conditionnement : 100ul
Marque : Genetex
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
![Anti-beta Tubulin antibody [D66] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11307](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-beta Tubulin antibody [D66] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11307")
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | D66 |
Isotype | IgG1 |
Application | WB, ICC/IF, IP, ELISA |
Reactivity | Human, Mouse, Rat, Rabbit, Bovine, Dog, Hamster, Chicken, Monkey, Chlamydomonas reinhardtii, Dinoflagellate, Sea Urchin |
| Package | 100 μl |
Datasheet File View product citations for antibody GTX11307 on CiteAb
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| WB | 0.5-1.0 μg/ml |
| ICC/IF | Assay dependent |
| IP | Assay dependent |
| ELISA | Assay dependent |
Not tested in other applications.
Calculated MW
50 kDa. ( Note )
Positive Control
PC12
Product Note
Recognizes all mouse brain β-tubulin isoforms (β and β′ subpopulations), and sea urchin β2-tubulin. The epitope recognized by the antibody is localized in the C-terminal region of β-tubulin (amino acids 427-432 in sea urchin β2-tubulin).
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
~1? mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Sea Urchin
Immunogen
sea urchin (Lytechinus pictus) sperm axonemal proteins.
Purification
Purified immunoglobulin
Conjugation
Unconjugated
RRID
AB_372176
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tubulin beta class I , CDCBM6 , CSCSC1 , M40 , OK/SW-cl.56 , TUBB1 , TUBB5
Cellular Localization
Cytoplasm, cytoskeleton
Background
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.
Database
DATA IMAGES
| | GTX11307 ICC/IF ImageICC/IF analysis of SH-SY-5Y cells using GTX11307 beta Tubulin antibody [D66] at 0.5 μg/mL(red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone. |
| | GTX11307 WB ImageWB analysis of (1) HeLa, (2) U87, (3) P19, (4) Neuro-2a, (5) PC-12, (6) COS-7, (7) CHO, (8) MDBK and (9) MDCK lysates using beta Tubulin antibody [D66] at of 1 μg/ml. |
| | GTX11307 WB ImageThe data was published in the journal Cancer Med in 2019. PMID: 31274246 |
| | GTX11307 ICC/IF ImageThe data was published in the journal Cancer Med in 2019. PMID: 31274246 |
| | GTX11307 ICC/IF ImageThe data was published in the journal Cell Adh Migr in 2019.PMID: 30744493 |
Application Reference
Lee WJ et al. Cancer Med 2019; 8 (10) : 4821-4835 Melatonin promotes neuroblastoma cell differentiation by activating hyaluronan synthase 3-induced mitophagy.
Application : WB
Reactivity : Mouse
Lin JH et al. Cell Adh Migr 2019; : 1-18 Small G protein signalling modulator 2 (SGSM2) is involved in oestrogen receptor-positive breast cancer metastasis through enhancement of migratory cell adhesion via interaction with E-cadherin.
Application : ICC/IF
Reactivity : Human
Juenemann K et al. Neurotox Res 2011; 20 (2) : 120-33 Modulation of mutant huntingtin N-terminal cleavage and its effect on aggregation and cell death.
Application : WB
Reactivity : Human
REVIEW
Application Reference
Lee WJ et al. Cancer Med 2019; 8 (10):4821-4835 Melatonin promotes neuroblastoma cell differentiation by activating hyaluronan synthase 3-induced mitophagy.
Application : WB
Reactivity : Mouse
Lin JH et al. Cell Adh Migr 2019;:1-18 Small G protein signalling modulator 2 (SGSM2) is involved in oestrogen receptor-positive breast cancer metastasis through enhancement of migratory cell adhesion via interaction with E-cadherin.
Application : ICC/IF
Reactivity : Human
Juenemann K et al. Neurotox Res 2011; 20 (2):120-33 Modulation of mutant huntingtin N-terminal cleavage and its effect on aggregation and cell death.
Application : WB
Reactivity : Human
| SDS | |
|---|---|
| PBS.pdf | |
| Sodium Azide.pdf | |
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