Abx090811-1000ml | Blocking Buffer and Coating Stabilizer Clinisciences

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Datasheet SDS

Specially formulated reagent to improve the stability and function of antigens/proteins bound to a solid phase. Use as supplied for very unstable proteins or for less labile antigens/proteins dilute up to 1:1 in your current blocker.

Target	Coating Stabilizer & Blocking Buffer
Conjugation	Unconjugated
Form	Liquid
Purification	0.2 µm filtered.
Storage	Store at 2-8 °C. Do not freeze.
Buffer	pH 7.2 ± 0.2. Contains a proprietary preservative (a non-mercury and azide free active ingredient against micro-organisms).
Specificity	This product is a specially formulated reagent to improve the stability and function of antigens/proteins bound to a solid phase. Use as supplied for very unstable proteins or less labile antigens/proteins, dilute up to 1:1 in your current blocker.
Availability	Shipped within 10-20 working days.
Note	This product is for research use only.
Directions for use	Centrifuge before opening to ensure complete recovery of vial contents. Recommended Protocol for Stabilizing and Blocking Immobilised Proteins: After coating the surface with protein/antigen, wash once to remove excess and weakly adsorbed protein. Before the protein begins to dry, coat the surface with the Coating Stabilizer and Blocking Buffer to completely cover the bound material. Allow to incubate at room temperature for 15 to 60 minutes. Aspirate or drain the excess stabilizer from the surface. Do not wash the surface. Dry the protein, preferably under vacuum. Recommended drying times are as follows: Two hours under vacuum (< 100 micron). Overnight in a humidity controlled chamber that registers < 15% humidity Package the bound antigen/protein in a sealed airtight container with desiccant. The product is now stabilized for long-term storage at 2–8 °C.

Research Articles on Coating Stabilizer & Blocking Buffer

Blocking Buffer and Coating Stabilizer

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