BPTF/FALZ TR-FRET, BioAssay™ Kit

Référence 298337-1Kit

Conditionnement : 1Kit

Marque : US Biological

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298337 BPTF/FALZ TR-FRET, BioAssay™ Kit

Clone Type
Polyclonal
Swiss Prot
Q12830
Shipping Temp
Blue Ice
Storage Temp
-20°C/-70°C

Kit comes in a convenient format with purified BPTF/FALZ, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.||The BPTF TR-FRET BioAssay™ Kit is designed to measure the inhibition of the binding of BPTF, also known as FALZ, to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BPTF, substrate, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.||Applications: |Suitable for use in screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested.||Kit Components:|White Nonbinding low volume microtiter plate (Corning), 1x plate|BPTF(FALZ), GST-tag, 1x10ug|BET Bromodomain Ligand, 1x75ul |Non-acetylated Ligand 1, 1x15ul|Tb donor, 1x20ul |Dye-labeled acceptor, 1x20ul |3x BPTF TR-FRET Assay Buffer, 1x4ml ||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Filippakopoulos, P., et al., Cell 2012; 149:214.