Caspase 5 (CASP5) (202-419) Mouse Monoclonal Antibody [Clone ID: 4F7]
CAT#: AM26609AF-N
Caspase 5 (CASP5) (202-419) mouse monoclonal antibody, clone 4F7, Azide Free
Specifications
Product Data | |
Clone Name | 4F7 |
Applications | WB |
Recommended Dilution | Western blot: 1 µg/ml for chemiluminescence detection system. For detatil s see protocol below. |
Reactivities | Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Recombinant C-terminal region of caspase-5 (202-419 a.a.) |
Specificity | This antibody reacts with 47 kDa of caspase-5. |
Formulation | PBS containing 50% glycerol, pH 7.2. Contains no preservatives. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | caspase 5 |
Database Link | |
Background | Apoptosis is a major form of cell death characterized by morphological features including chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies. These morphological changes occur via signaling pathways that lead to the recruitment and activation of caspases, a family of cysteine-containing, aspartate-specific proteases. Caspases exist as inactive proenzymes in cells and are activated through their processing into two subunits in response to apoptotic stimulation. Activated caspases cleave a variety of important cellular proteins, other caspases, and Bcl-2 family members, leading to a commitm ent to cell death. Caspase-5 (also known as ICE rel -III or ICH-3) is a ~47 kDa member of the ICE/CED-3 family of cysteine proteases. This protein has 51% sequence identity with human interleukin-1- β - converting enzyme, 27% sequence identity with ICH-1 L, 30% identity with human CPP32, and 24% sequence identity with the C. elegans CED-3 polypeptide. This ICE subfamily of caspase includes caspase-1, caspase-5, caspase-4, and caspase-13, and appears to play a primary role in cytokine maturation and inflammation. |
Synonyms | CASP-5, CASP5, ICH3, ICH-3 protease, TY protease, ICE(rel)-III |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting |
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