Caspase 8 (CASP8, CASP-8, ALPS2B, Apoptotic Cysteine Protease, Apoptotic Protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like Protease, FADD-like ICE, FLICE, FLJ17672, ICE-like Apoptotic Protease 5, MORT1-associated Ced-3 Homolog, MACH, MCH5, MGC78473) (Biotin)

Référence C2087-33D-100ul

Conditionnement : 100ul

Marque : US Biological

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C2087-33D Caspase 8 (CASP8, CASP-8, ALPS2B, Apoptotic Cysteine Protease, Apoptotic Protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like Protease, FADD-like ICE, FLICE, FLJ17672, ICE-like Apoptotic Protease 5, MORT1-associated Ced-3 Homolog, MACH, MCH5, MGC78473) (Biotin)

Clone Type
Polyclonal
Host
mouse
Source
human
Isotype
IgG1
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mk
Accession #
NP_001219.2; NP_203519.1; NP_203520.1
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-8 (FLICE) forms a direct link between the activation of CD95 and the caspase pathway. Overexpression of Caspase-8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family.||Applications:|Suitable for use in ELISA and Western Blot. Other applications not tested.||Recommended Dilution:|Western Blot: 0.5-2ug/ml|ELISA: 0.5-2ug/ml|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 11E49 (90A992)|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.|Purity: Purified by Protein G affinity chromatography.|Immunogen: Synthetic peptide corresponding to aa217-222 (SESQTC) of human Caspase-8.|Specificity: Recognizes the pro-form of human Caspase-8. Species Crossreactivity: chimpanzee, rhesus monkey.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to aa217-222 (SESQTC) of human Caspase-8.
Form
Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes the pro-form of human Caspase-8. Species Crossreactivity: chimpanzee, rhesus monkey.
References
1. Hirokawa S, Sato H, Kato I, Kudo A. EBF-regulating Pax5 transcription is enhanced by STAT5 in the early stage of B cells. Eur J Immunol. 2003 Jul;33(7):1824-9. 2. Cotta CV, Zhang Z, Kim HG, Klug CA. Pax5 determines B- versus T-cell fate and does not block early myeloid-lineage development. Blood. 2003 Jun 1;101(11):4342-6. Epub 2003 Jan 30.