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> CD14 (Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein) (PE)

CD14 (Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein) (PE)

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Référence C2265-35N-100T

Conditionnement : 100Tests

Marque : US Biological

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C2265-35N CD14 (Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein) (PE)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P08571
Isotype
IgG2a
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo Ca Fe Gt Hu Mk Po Rb Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD14 is a cell surface antigen. It is expressed strongly by most peripheral blood monocytes and weakly on granulocytes.||Applications:|Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilutions:|Flow Cytometry: Neat-1:10, Use 10ul to label 10e6 cells or 100ul whole blood.|Optimal dilutions to be determined by researcher.||Storage and Stability:|Store product at 4°C before and after reconstitution. DO NOT FREEZE! Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Further dilutions can be made in assay buffer. Aliquots are stable for 12 months after receipt. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 3H501 (TuK4)|Host: mouse|Source: human|Concentration: Not Determined|Form: Supplied as a lyophilized powder from PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O. |Purity: Purified by Protein A affinity chromatography|Specificity: Recognizes human CD14. Species Crossreactivity: canine, goat, feline, rabbit, mink, bovine, porcine, sheep, Cynomolgus monkey and llama.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder from PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Purity
Purified by Protein A affinity chromatography
Specificity
Recognizes human CD14. Species Crossreactivity: canine, goat, feline, rabbit, mink, bovine, porcine, sheep, Cynomolgus monkey and llama.
References
1. Yoshino, N., et al., Exp. Anim. 49(2): 97-110 (2000). 2. Jacobson, C.N., et al., Vet. Immunol. Immunopath. 39: 461-466 (1993). 3. Sopp, P., et al., Vet. Immunol. Immunopath. 56: 11-25 (1997). 4. Arriens, M.A., et al., Vet. Immunol. Immunopath. 54: 113 (1996). 5. Willett, B., et al., J. Virol. 77(1): 709-712 (2003). 6. Yang, H., et al., Immunol. Letters 88: 27-30 (2003). 7. Soell, M., et al., J. Immunol. 154: 851-860 (1995). 8. Gupta, V.K., et al., Vet. Immunol. Immunopathol. 51: 89-99 (1996). 9. Werling, D., et al., Vet. Immunol. Immunopathol. 62: 185-195 (1998). 10. Dalli, J. et al. (2008) Blood. 112: 2512­2519. 11. Lybeck, K.R. et al. (2009) Clin. Vaccine. Immunol. 16: 1003­11. 12. Ferret­Bernard, S. et al. (2010) PLoS One. 5: e13705. 13. Fulton, B.E. Jr. et al. (2006)J Virol. 80: 7873­84. 14. Willett, B.J. et al. (2007) J Virol. 81: 9665­79. 15. Kallapur, S.G. et al. (2011) Am J Physiol Lung Cell Mol Physiol. Jun 10. [Epub ahead of print]