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> CD205 (DEC205, WC6) (PE)

CD205 (DEC205, WC6) (PE)

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Référence 214605-PE-100ul

Conditionnement : 100ul

Marque : US Biological

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214605-PE CD205 (DEC205, WC6) (PE) discontinued

Clone Type
Polyclonal
Host
mouse
Source
bovine
Isotype
IgG1
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Bo Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do not freeze

CD205 (also known as DEC205) and originally described as BoWC6. CD205 is a cell surface antigen with an approximate molecular weight of 210-220kD.|CD205 is expressed on afferent lymph dendritic cells (veiled cells), B cells, a subset of T cells and by cortical and medullary thymocytes.||Applications: |Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Immunohistochemistry: Frozen sections|Optimal dilutions to be determined by the researcher.||Hybridoma: |X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: IL-A114|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Specificity: Recognizes bovine CD205. Species Crossreactivity: sheep||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes bovine CD205. Species Crossreactivity: sheep
References
1. Dutia, B.M. et al. (1993) Analysis of the monoclonal antibodies comprising WC6. Vet. Immunol. Immunopathol. 39: 193-199. 2. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants. Vet. Immunol. Immunopathol. 39: 283-290. 3. Gliddon, D. R. et al. (2004) DEC-205 expression on migrating cells in afferent lymph. Immunology 111:262-272. 4. Parsons, K.R. et al. (1993) Studies of monoclonal antibodies indentifying two novel bovine lymphocyte antigen differentiation clusters: workshop clusters (WC) 6 and 7. Vet. Immunol. Immunopathol. 39: 187-192. 5. Howard, C.J. et al. (1993) Summary of workshop findings for cattle (Tables 1 and 2). Vet. Immunol. Immunopathol. 39: 25-48. 6. Tamao, H. et al. (2011) Distribution of immune cells and expression of interleukin receptors in ileal Peyer's patches of calves. Cell Tissue Res. 346: 245-54.