Images
Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of human activated T lymphocytes with CD44 recombinant monoclonal antibody, clone Hermes-1 (Cat # RAB03282).
Human activated T lymphocytes were stained with an isotype control (3.0- panel A) or the rmeric version of RAB03282 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against human CD44.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against human tonsil lymphocytes.

    Reactivity

    Bovine, Goat, Human, Mouse, Primates, Rabbit, Rat, Sheep

    Form

    Liquid

    Isotype

    IgG kappa

    Recommend Usage

    Blocking
    ELISA
    Flow Cytometry
    Immunocytochemistry
    Immunofluorescence
    Immunohistochemistry
    Immunoprecipitation
    Neutralization
    Western Blot
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.02% Proclin 300)

    Storage Instruction

    Store at 4°C for 3 months. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

    Immunohistochemistry

    Immunocytochemistry

    Immunofluorescence

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

    Blocking

    Flow Cytometry

    Flow cytometric analysis of human activated T lymphocytes with CD44 recombinant monoclonal antibody, clone Hermes-1 (Cat # RAB03282).
    Human activated T lymphocytes were stained with an isotype control (3.0- panel A) or the rmeric version of RAB03282 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer.

    Neutralization

  • Gene Info — CD44

    Entrez GeneID

    960

    Gene Name

    CD44

    Gene Alias

    CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1

    Gene Description

    CD44 molecule (Indian blood group)

    Omim ID

    107269

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq

    Other Designations

    CD44 antigen|CD44 antigen (homing function and Indian blood group system)|CDW44 antigen|GP90 lymphocyte homing/adhesion receptor|Hermes antigen|antigen gp90 homing receptor|cell adhesion molecule|cell surface glycoprotein CD44|chondroitin sulfate proteogl

  • Interactomes
  • Pathways
  • Diseases