Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1
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- More Files
Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibodody, clone 84-1. Western Blot analysis of VIM expression in MCF-7.
Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibodody, clone 84-1. Western Blot analysis of VIM expression in SK-BR-3.
Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibodody, clone 84-1. Western Blot analysis of VIM expression in MDA-MB-231.
Immunofluorescence
Immunofluorescence staining on non-fixed, non-permeabilized MDA-MB-231 cells using FITC conjugated Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1 for CSV (Green) and DAPI for nucleus (Blue).
Immunofluorescence
Immunofluorescence staining of CSV on isolated LM7 cancer cells and leukocytes using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. The cells were co-stained against DRAQ5, CSV and CD45. (Satelli A., et al. Cancer Research 2014; 74:1645-1650)
Flow Cytometry
Flow cytometric analysis of CSV expression in cancer (top) and normal (bottom) cell lines using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Isotype controls were used as negative controls. (Satelli A., et al., Clinical Cancer Research 2014; 21(4):899-906)
- Specifications
Product Description
Mouse monoclonal antibody recognizes human cell-surface vimentin (CSV).
Immunogen
Human recombinant vimentin
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Form
Liquid
Isotype
IgG2b, kappa
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
Cell-Surface Vimentin (CSV) detecting antibody is best used before cell fixation and permeabilization. If fixation is required, please use Abnova’s Special Fixative.
Cell-Surface Vimentin (CSV) antibody is a pending MD Anderson patent which has been exclusively licensed to Abnova Corporation. - Applications
Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibodody, clone 84-1. Western Blot analysis of VIM expression in MCF-7.Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibodody, clone 84-1. Western Blot analysis of VIM expression in SK-BR-3.Western Blot (Cell lysate)
Cell-Surface Vimentin (CSV) monoclonal antibodody, clone 84-1. Western Blot analysis of VIM expression in MDA-MB-231.Immunofluorescence
Immunofluorescence staining on non-fixed, non-permeabilized MDA-MB-231 cells using FITC conjugated Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1 for CSV (Green) and DAPI for nucleus (Blue).Immunofluorescence
Immunofluorescence staining of CSV on isolated LM7 cancer cells and leukocytes using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. The cells were co-stained against DRAQ5, CSV and CD45. (Satelli A., et al. Cancer Research 2014; 74:1645-1650)Flow Cytometry
Flow cytometric analysis of CSV expression in cancer (top) and normal (bottom) cell lines using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Isotype controls were used as negative controls. (Satelli A., et al., Clinical Cancer Research 2014; 21(4):899-906) - Gene Info — VIM
Entrez GeneID
7431Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
- Interactomes
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- Publication Reference
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Application：IF, Human, MCF-7, MCF-7/PMA+ TGF-β, MDA-MB-231 cells.
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