COLGALT1 ELISA Kit (Human)

Référence OKEH04710

Conditionnement : 96Wells

Marque : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology COLGALT1 ELISA Kit (Human) (OKEH04710) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COLGALT1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COLGALT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample COLGALT1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
COLGALT1 Microplate96 Wells (12 x 8 Well Strips)
COLGALT1 Standard2
Sample Diluent1 x 20 mL
COLGALT1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.05 ng/mL
SpecificityNatural and recombinant Human Procollagen galactosyltransferase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCOLGALT1
Gene Full Namecollagen beta(1-O)galactosyltransferase 1
Alias SymbolsBSVD3;ColGalT 1;Collagen beta(1-O)galactosyltransferase 1;GLT25D1;glycosyltransferase 25 domain containing 1;glycosyltransferase 25 family member 1;hydroxylysine galactosyltransferase 1;procollagen galactosyltransferase 1.
NCBI Gene Id79709
Protein NameQ8NBJ5
Description of TargetProcollagen galactosyltransferase 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q8NBJ5
Protein Accession #NP_078932.2
Nucleotide Accession #NM_024656.3
Protein Size (# AA)622
Molecular Weight72 kDa

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