Concanavalin A, CF®680 Conjugate
Référence 29020
Conditionnement : 5x1MG
Marque : Biotium
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Concanavalin A (Con A) CF® Dye Conjugates
A lectin labeled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.
Technology
Cellular Stains
CF® Dye & Other Bioconjugates
CF® Dyes
Membrane & Cell Surface Stains Comparison
Protocols
Supporting Documents
Product Description
Concanavalin A (Con A) is a widely used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are commonly found in the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.
- Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
- Detect glycoconjugates in microscopy and flow cytometry
- Stain glycoproteins in gels
- Withstands fixation and permeabilization
- Choice of 10 CF® dyes from UV to near-infrared
- Superior CF® dyes are bright, photostable, and water-soluble
Lectins are also versatile probes for detecting glycoconjugates in microscopy and flow cytometric applications and for gel staining of glycoproteins. In neutral and alkaline solutions, Con A exists as a tetramer with a molecular weight of approximately 104 kDa. In acidic solutions (pH below 5.0), Con A exists as a dimer. Con A can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway.
Find the Right Stain for Your Application
Con A and other lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins. The presence and distribution of these targets vary between cell types and tissues. As a result, other cell surface stains or other lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, may produce better surface staining and may be more appropriate for your cell type. Lectin conjugates can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway. Lectins may be toxic or stimulatory to live cells depending on cell type. To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Superior CF® Dyes
Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.
Concanavalin A Conjugates
| Product | Conjugation | Ex/Em | Size | Catalog No. | Purchase |
|---|---|---|---|---|---|
| CF®350 Concanavalin A (Con A) | CF®350 | 347/448 nm | 5 mg | 29015 | Purchase 29015 |
| CF®405S Concanavalin A (Con A) | CF®405S | 404/431 nm | 5 mg | 29075 | Purchase 29075 |
| CF®405M Concanavalin A (Con A) | CF®405M | 408/452 nm | 5 mg | 29074 | Purchase 29074 |
| CF®488A Concanavalin A (Con A) | CF®488A | 490/515 nm | 5 mg | 29016 | Purchase 29016 |
| CF®594 Concanavalin A (Con A) | CF®594 | 593/614 nm | 5 mg | 29017 | Purchase 29017 |
| CF®633 Concanavalin A (Con A) | CF®633 | 630/650 nm | 5 mg | 29018 | Purchase 29018 |
| CF®640R Concanavalin A (Con A) | CF®640R | 642/662 nm | 5 mg | 29019 | Purchase 29019 |
| CF®680 Concanavalin A (Con A) | CF®680 | 681/698 nm | 5 mg | 29020 | Purchase 29020 |
| CF®750 Concanavalin A (Con A) | CF®750 | 755/777 nm | 5 mg | 29080 | Purchase 29080 |
| CF®770 Concanavalin A (Con A) | CF®770 | 770/797 nm | 5 mg | 29058 | Purchase 29058 |
Full List of Lectin Conjugates
| Product | Features |
|---|---|
| CF® Dye Concanavalin A (Con A) | • Cell surface stain for yeast, fungi, and mammalian cells • Selectively binds to a-mannopyranosyl and a-glucopyranosy residues • Available with a wide selection of CF® Dyes |
| CF® Dye Wheat Germ Agglutinin (WGA) | • Cell surface stain for mammalian cells and gram+ bacteria • Also stains yeast bud scars • Has high affinity for sialic acid and N-acetylglucosamine • Choose from a wide selection of CF® Dyes or HRP |
| CF® Dye Peanut Lectin (PNA) from Arachis hypogaea | • Specific for terminal β-galactose and binds preferentially to galactosyl (β-1,3) N-acetylgalactosamine • Choice of 4 CF® dye colors |
| CF® Dye Lycopersicon Esculentum (Tomato) Lectin (LEL, TL) | • Marker for blood vessels and microglial cells • Binds to [GlcNAc] 1,3-N-acetylglucosamine, glycophorin, and Tamm-Horsfall glycoprotein • Used to study tumor angiogenesis or tracing neovascular development in xenograft models • Choice of 5 CF® Dyes or biotin |
| CF® Dye Ulex Europaeus Agglutinin I (UEA I) | • Marker for human endothelial cells and incompletely differentiated gastrin cells • Binds to glycoproteins and glycolipids containing α-linked fucose residues • Choice of 5 CF® Dyes or biotin |
| CF® Dye Phaseolus Vulgaris Leucoagglutinin (PHA-L) | • Used to stimulate lymphocyte and T cell proliferation • Choice of 5 CF® Dyes or biotin |
| CF® Dye Datura Stramonium Lectin (DSL) | • Binds to (beta-1,4) linked N-acetylglucosamine oligomers • Choice of 5 CF® Dyes or biotin |
| CF® Dye Sambucus Nigra Lectin (SNA, EBL)) | • Binds to sialic acid attached to terminal galactose • Choice of 6 CF® Dyes or biotin |
CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.
References
CF®680 ConA
Acta Histochem (2019), DOI: 10.1016/j.acthis.2018.11.006.
Download a list of CF® dye references.