Creatinine Serum Kit
Référence OKAU00065-2PLATE
Conditionnement : 2plate
Marque : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for Creatinine Serum Kit (OKAU00065) |
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| Application | ELISA-Sandwich | ||||||||||||||||||||||||||||||
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| ELISA Kit Linearity | Linearity was determined by taking two human serum samples, one with a low diluted creatinine level of 0.75 mg/dL and one with a higher level of 3.78 mg/dL and mixing them in ratios given below. The measured concentrations were compared to the expected values.
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| ELISA Kit Principle | The DetectX® Serum Creatinine Kits are designed to quantitatively measure creatinine present in serum samples. Please read the complete kit insert before performing this assay. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the DetectX® Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read after 1 minute in a microtiter plate reader capable of measuring 490nm wavelength. At 30 minutes the optical density is read again. The concentration of creatinine is calculated using the delta of the optical density readings at 30 and 1 minute compared to the curve generated from the standards, or by using the Excel worksheet available for free download at our web site. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum. | ||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra Assay Precision Three human serum samples were run in replicates of 20 in an assay. The mean and precision of the calculated creatinine concentrations were:
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| Additional Information | Background: Creatinine (2-amino-1-methyl-5H-imidazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Creatinine forms spontaneously from p-creatine. Under normal conditions, its formation occurs at a rate that is relatively constant and as intra-individual variation is < 15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally, altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease. | ||||||||||||||||||||||||||||||
| :: | Cross Reactivity: It is well known that some typical components of serum may interfere with the Jaffe reaction for creatinine measurement. | ||||||||||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||||||||||||||
| Sample Type | Mammalian Serum and Plasma | ||||||||||||||||||||||||||||||
| Sensitivity | 0.081 mg/dL |
| Gene Full Name | Creatinine |
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