CRMP5 (DPYSL5) (NM_020134) Human Tagged ORF Clone
Référence RC202631
Conditionnement : 10ug
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| Product Data | |
| Target Symbol | CRMP5 |
|---|---|
| Synonyms | CRAM; CRMP-5; CRMP5; CV2; Ulip6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >RC202631 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGCCAACTCAGCCAGCGTGAGGATCCTCATCAAGGGAGGCAAGGTGGTGAACGATGACTGCACCC ACGAGGCTGACGTCTACATCGAGAATGGCATCATCCAGCAGGTGGGCCGCGAGCTCATGATCCCTGGCGG GGCCAAGGTGATTGATGCCACAGGAAAACTGGTGATCCCTGGTGGCATCGACACCAGCACCCACTTCCAC CAGACCTTCATGAATGCCACGTGCGTGGACGACTTCTACCATGGGACCAAGGCAGCACTCGTCGGAGGCA CCACCATGATCATCGGCCACGTCCTGCCCGACAAGGAGACCTCCCTTGTGGACGCTTATGAGAAGTGCCG AGGTCTGGCCGACCCCAAGGTCTGCTGTGATTACGCCCTCCACGTGGGGATCACCTGGTGGGCACCCAAG GTGAAAGCAGAAATGGAGACACTGGTGAGGGAGAAGGGTGTCAACTCGTTCCAGATGTTCATGACCTACA AGGACCTGTACATGCTTCGAGACAGTGAGCTGTACCAAGTGTTGCACGCTTGCAAGGACATTGGGGCAAT CGCCCGCGTCCATGCTGAAAATGGGGAGCTTGTGGCCGAGGGTGCTAAGGAGGCACTGGATTTGGGGATC ACAGGCCCAGAAGGAATCGAGATCAGCCGTCCAGAGGAGCTGGAAGCTGAAGCCACTCATCGTGTTATCA CCATTGCAAACAGGACTCACTGTCCAATCTACCTGGTCAACGTGTCCAGTATCTCGGCTGGTGACGTTAT CGCAGCTGCTAAGATGCAAGGGAAGGTTGTGCTGGCGGAGACCACCACTGCACATGCCACGCTGACAGGC TTACACTACTACCACCAGGACTGGTCCCACGCGGCTGCCTATGTCACGGTGCCTCCCCTGAGACTGGACA CCAACACCTCAACCTACCTCATGAGCCTGCTGGCCAATGACACTCTGAACATCGTGGCATCAGATCACCG GCCTTTCACCACAAAGCAGAAAGCTATGGGCAAGGAAGACTTCACCAAGATCCCACATGGAGTGAGTGGC GTGCAGGACCGCATGAGCGTCATCTGGGAGAGAGGAGTGGTTGGAGGAAAGATGGATGAGAACCGTTTTG TGGCCGTTACCAGTTCCAACGCAGCTAAGCTTCTGAACCTGTATCCCCGCAAGGGCCGCATTATTCCCGG AGCCGATGCTGATGTGGTGGTGTGGGACCCAGAAGCCACAAAGACCATCTCAGCCAGCACGCAGGTCCAG GGAGGAGACTTCAACCTGTATGAGAACATGCGCTGCCACGGCGTGCCACTGGTCACCATCAGCCGGGGGC GCGTCGTGTATGAGAACGGCGTCTTCATGTGCGCCGAGGGCACCGGCAAGTTCTGTCCCCTGAGGTCCTT CCCAGACACTGTCTACAAGAAGCTGGTCCAGAGAGAGAAGACTTTAAAGGTTAGAGGAGTGGACCGCACT CCCTACCTGGGGGATGTCGCTGTTGTCGTGCACCCTGGGAAAAAAGAGATGGGAACCCCACTCGCAGACA CTCCTACCCGGCCCGTCACCCGGCATGGGGGCATGAGGGACCTTCACGAATCCAGCTTCAGCCTCTCTGG CTCTCAGATCGATGACCATGTTCCAAAGCGAGCTTCAGCTCGGATCCTCGCTCCTCCCGGAGGCAGGTCG AGTGGCATTTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC202631 protein sequence Red=Cloning site Green=Tags(s) MLANSASVRILIKGGKVVNDDCTHEADVYIENGIIQQVGRELMIPGGAKVIDATGKLVIPGGIDTSTHFH QTFMNATCVDDFYHGTKAALVGGTTMIIGHVLPDKETSLVDAYEKCRGLADPKVCCDYALHVGITWWAPK VKAEMETLVREKGVNSFQMFMTYKDLYMLRDSELYQVLHACKDIGAIARVHAENGELVAEGAKEALDLGI TGPEGIEISRPEELEAEATHRVITIANRTHCPIYLVNVSSISAGDVIAAAKMQGKVVLAETTTAHATLTG LHYYHQDWSHAAAYVTVPPLRLDTNTSTYLMSLLANDTLNIVASDHRPFTTKQKAMGKEDFTKIPHGVSG VQDRMSVIWERGVVGGKMDENRFVAVTSSNAAKLLNLYPRKGRIIPGADADVVVWDPEATKTISASTQVQ GGDFNLYENMRCHGVPLVTISRGRVVYENGVFMCAEGTGKFCPLRSFPDTVYKKLVQREKTLKVRGVDRT PYLGDVAVVVHPGKKEMGTPLADTPTRPVTRHGGMRDLHESSFSLSGSQIDDHVPKRASARILAPPGGRS SGIW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_020134 |
| ORF Size | 1692 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@clinisciences.com The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020134.4 |
| RefSeq Size | 5225 bp |
| RefSeq ORF | 1695 bp |
| Locus ID | 56896 |
| UniProt ID | Q9BPU6 |
| Cytogenetics | 2p23.3 |
| Domains | Amidohydro_1 |
| Protein Pathways | Axon guidance |
| MW | 61.4 kDa |
| Summary | This gene encodes a member of the CRMP (collapsing response mediator protein) family thought to be involved in neural development. Antibodies to the encoded protein were found in some patients with neurologic symptoms who had paraneoplastic syndrome. A pseudogene of this gene is found on chromosome 11. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011] |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC202631L3 | Lenti ORF clone of Human dihydropyrimidinase-like 5 (DPYSL5), Myc-DDK-tagged | 10 ug | | |
| RC202631L4 | Lenti ORF clone of Human dihydropyrimidinase-like 5 (DPYSL5), mGFP tagged | 10 ug | | |
| RG202631 | DPYSL5 (tGFP-tagged) - Human dihydropyrimidinase-like 5 (DPYSL5) | 10 ug | | |
| SC113189 | DPYSL5 (untagged)-Human dihydropyrimidinase-like 5 (DPYSL5) | 10 ug | | |
| SC320721 | DPYSL5 (untagged)-Human dihydropyrimidinase-like 5 (DPYSL5) | 10 ug | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.