Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice. Recommended working dilutions: vary widely, but may be between 1/1000 and 1/9000.
Reactivity
Bovine
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Diaphorase isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity
IgG fraction of polyclonal rabbit antiserum to diaphorase from porcine heart. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Buffer
PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized IgG fraction
Reconstitution Method
Restore by adding 1.0 ml of sterile distilled water
Concentration
lot specific
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation
Unconjugated
Storage
Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
