CYP27A1 Human qPCR Primer Pair (NM_000784)
CAT#: HP200725
qSTAR qPCR primer pairs against Homo sapiens gene CYP27A1
SensiMix SYBR Master Mix
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Specifications
Product Data | |
Gene ID | 1593 |
Forward Sequence | GTGCTGCCTTTCTGGAAGCGAT |
Reverse Sequence | TAGCCAGACACCTGGATGCCAT |
Accession No | NM_000784, NM_000784.1, NM_000784.2, NM_000784.3, BC017044, BC040430, BC051851, NM_000784.4 |
UniProt ID | Q02318 |
Synonyms | CP27; CTX; CYP27 |
Component | 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM. |
Quality Control | The primer mix has been tested to generate satisfactory qPCR data on ABI 7900HT by using the following PCR program: Stage 1: Activation: 50 °C for 2 min; Stage 2: pre-soak:95 °C for 10 min; Stage 3: Denaturation: 95 °C for 15 sec, Annealing: 60°C for 1 min; Stage 4: Melting curve: 95°C for 15 sec, 60°C for 15 sec, 95°C for 15 sec. |
Storage | Store at -20°C. |
Stability | The primer mix is stable for one year from date of shipping. |
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