K2213-100 assays | D-Lactate Colorimetric Assay Kit Clinisciences

Background

Lactate is chiral: L(+)-Lactate and D(-)-Lactate. L(+)-Lactate exists in blood and is constantly produced from pyruvate by lactate dehydrogenase (LDH) in human intermediary metabolism. D-Lactate is mainly produced through the glyoxalase pathway in mammals. D(-)-Lactate is present only at about 1-5% of the concentration of L(+)-Lactate and is extremely low with normal serum concentrations in the nM to µM range. The elevated D-lactate levels which can rise to mM levels are caused by short bowel syndrome or bacterial infection in humans. Abnormal high concentration of D-lactate is related to diseases such as sepsis, ischemia or trauma. Because of slow metabolism and excretion, high D-lactate can cause acidosis and encephalopathy.

The D-Lactate Colorimetric Assay Kit provides a simple, fast and convenient way for accurate detection of D-lactate levels in various biological samples (serum, plasma, cells, culture and fermentation media) based on colorimetric method. In the assay, D-lactate is specifically oxidized by D-lactate dehydrogenase and produces proportional color (λmax = 450 nm). The kit can detect the useful D-lactate concentration range 0.01 mM-10 mM in various samples.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. 40 minutes; Kit contains all necessary reagents for accurate measurement of D-lactate.
Shipping	Blue Ice
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

D-Lactate Assay Buffer, D-Lactate Enzyme Mix, D-Lactate Substrate Mix, D-Lactate Standard (100 mM)

D-Lactate Colorimetric Assay Kit

Référence K2213-100 assays

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