Dimethylsulfoxide (DMSO) for Cell Culture

Référence D8050-01-50ml

Conditionnement : 50ml

Marque : US Biological

Demander plus d'informations

Contactez votre distributeur local :


Téléphone : +1 850 650 7790


D8050-01 Dimethylsulfoxide (DMSO) for Cell Culture

Clone Type
Polyclonal
Grade
Cell Culture Grade
Shipping Temp
RT
Storage Temp
RT

Ready-to-use sterile filtered product conveniently packaged in septum screw-capped amber bottles. ||DMSO has been used as a cryoprotectant and is still an important constituent of cryoprotectant vitrification mixtures used to preserve organs, tissues, and cell suspensions. Without it, up to 90% of frozen cells will become inactive. It is particularly important in the freezing and long-term storage of embryonic stem cells and hematopoietic stem cells, which are often frozen in a mixture of 10% DMSO, a freezing medium, and 30% fetal bovine serum. In the cryogenic freezing of heteroploid cell lines (MDCK, VERO, etc.) a mixture of 10% DMSO with 90% EMEM (70% EMEM + 30% fetal bovine serum + antibiotic mixture) is used.||Synonyms:|Methyl Sulfoxide; Dimethylsulfoxide; DMSO||CAS No:|67-68-5||Molecular Formula:|C2H6SO||Molecular Weight:|78.13||Purity (GC):|≥99%||Specific Gravity: |1.095-1.097||Congealing Temp: |18.3°C||Refractive Index: |1.4755-1.4780||Water:|≤0.1%||Titratable Acid: |≤5ml||Residue after Evaporation: |≤0.01%||Absorbance: |To Pass ACS Specifications||Substances Darkened by KOH: |To Pass ACS Specifications||RNase: |None Detected ||DNase: |None Detected||Endotoxin: |None Detected||Note: DMSO will congeal easily and may remain a solid at cool temperatures. Warm slightly to return to liquid state.

Applications
Important Note: |This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. ||Toxicity and Hazards: |All products should be handled by qualified personnel only, trained in laboratory procedures.
Form
Colorless, clear liquid at RT
Purity
≥99%
References
Pegg, DE. (2007). Day JG, Stacey GN, ed. "Principles of Cryopreservation". Cryopreservation and Freeze-Drying Protocols, Second Edition. Methods in Molecular Biology (Humana Press) 368: 39–57. doi:10.1007/978-1-59745-362-2_3. ISBN 978-1-58829-377-0. ISSN 1064-3745. PMID 18080461.

Vous serez peut-être également intéressé par les produits suivants :



Référence
Description
Cond.
Prix HT
0203-SC
 50ml 
0600-SC
 500ml