DNA Marker 50bp Ladder, 13 Fragments (50-1000bp), BioGenomics™

DNA Marker 50bp Ladder is designed for sizing and approximate quantification of wide range double-stranded DNA on agarose and polyacrylamide gels. The ladder is composed of 13 chromato- graphically purified individual DNA fragments (in base pairs): 1000, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100, 50.|Note: The bright bands at 500 and 250bp serve as the reference.||Concentration:|0.5mg DNA/ml||Storage Buffer:|10mM Tris-HCl (pH 7.6), 1mM EDTA||6X Loading Dye Solution:|Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA.||Quality Control:|Analysis of 0.5ug (1ul) of the DNA Ladder on agarose gel and 1ug (2ul)-on polyacrylamide gel by ethidium bromide staining generates 13 discrete band patterns.||Quantity of DNA in Band:|Fragment (bp) % ng/0.5ug DNA|1000 6 30|900 6 30|800 6 30 |700 6 30 |600 6 30 |500 15 75 |400 6 30 |300 6 30|250 15 75|200 7 35|150 7 35|100 7 35|50 7 35|Total 100 500||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.