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> Donkey IgG (H&L) antibody (Peroxidase)

Donkey IgG (H&L) antibody (Peroxidase)

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Référence orb348001-2mg

Conditionnement : 2mg

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    Donkey IgG (H&L) antibody (Peroxidase)

    Catalog Number: orb348001

    Catalog Numberorb348001
    CategoryAntibodies
    DescriptionDonkey IgG (H&L) antibody (Peroxidase)
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityDonkey
    IsotypeIgG
    ImmunogenDonkey IgG whole molecule
    Concentration2.0 mg/mL
    Dilution rangeELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
    Form/AppearanceLyophilized
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Donkey IgG and Donkey Serum.
    ConjugationHRP
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Alternative namesrabbit anti-Donkey IgG Antibody peroxidase Conjuga
    NoteFor research use only
    Application notesAnti-Donkey IgG Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
    Expiration Date12 months from date of receipt.
    Donkey IgG (H&L) antibody (Peroxidase)

    ELISA Results of Rabbit Anti-Donkey IgG Antibody Peroxidase Conjugated tested against purified Donkey IgG HRP. Each well was coated in duplicate with 1.0 µg of Donkey IgG. The working dilution is 1:93000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer and TMB substrate (p/n orb348651).