E-Cadherin antibody
Référence GTX100443-100ul
Conditionnement : 100ul
Marque : GeneTex
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Wm, IP, PLA |
Reactivity | Human, Mouse, Rat, Zebrafish, Dog |
Package | 100 μl, 25 μl |
Summary
APPLICATION
Application Note
Application | Recommended Dilution |
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WB | 1:500-1:10000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IHC-Wm | Assay dependent |
IP | 1:100-1:500 |
PLA | Assay dependent |
Calculated MW
Positive Control
Predict Reactivity
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Cellular Localization
Background
Database
DATA IMAGES
GTX100443 WB Image Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody (GTX100443) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100443 WB ImageNon-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100443 IHC-P ImageE-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100443 IHC-P ImageE-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog skin.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100443 ICC/IF ImageE-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500. |
GTX100443 WB ImageWhole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100443 IP Image E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments. |
GTX100443 ICC/IF ImageE-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm. |
GTX100443 IHC-P ImageImmunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody (GTX100443). |
GTX100443 IHC-P Image E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. |
GTX100443 IHC-P Image E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis. |
GTX100443 IHC-P Image E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. |
GTX100443 IHC-P Image E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis. |
GTX100443 ICC/IF Image E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. |
GTX100443 IHC-P Image E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis. |
GTX100443 IHC-P Image E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis. |
GTX100443 IHC-P ImageE-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100443 ICC/IF Image E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. |
GTX100443 ICC/IF ImageE-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500. |
GTX100443 IHC-P ImageE-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100443 IP Image Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody (GTX100443). |
GTX100443 WB ImageMouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100443 WB ImageVarious whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100443 WB ImageThe data was published in the 2022 in Front Pharmacol. PMID: 35517780 |
GTX100443 WB ImageThe data was published in the journal Front Oncol in 2020.PMID: 32211324 |
GTX100443 WB ImageThe data was published in the journal PLoS One in 2017. PMID: 28683123 |
GTX100443 WB ImageThe data was published in the journal PLoS One in 2017. PMID: 28683123 |
GTX100443 WB ImageThe data was published in the journal Sci Rep in 2017. PMID: 28195146 |
GTX100443 WB ImageThe data was published in the journal Front Endocrinol (Lausanne) in 2017. PMID: 29109696 |
GTX100443 WB ImageThe data was published in the 2022 in Front Pharmacol. PMID: 35517780 |
GTX100443 WB ImageThe data was published in the 2022 in Front Pharmacol. PMID: 35517780 |
GTX100443 WB ImageThe data was published in the 2022 in Front Pharmacol. PMID: 35517780 |
GTX100443 IHC-P ImageThe data was published in the journal Cell Death Dis in 2020.PMID: 32080178 |
GTX100443 WB ImageThe data was published in the journal Int J Mol Sci in 2019.PMID: 31426484 |
GTX100443 IHC-P ImageThe data was published in the journal Evid Based Complement Alternat Med in 2019. PMID: 31186661 |
Application Reference
REVIEW
E-Cadherin antibody Cat. No. GTX100443
Rating | | ( Average 4.8 based on 9 users reviews) |
Western Blot(WB) | | ( Average 4.7 based on 6 users reviews) |
Immunocytochemistry/ Immunofluorescence(ICC/IF) | | ( Average 5 based on 1 users reviews) |
IHC (Paraffin sections)(IHC-P) | | ( Average 5 based on 2 users reviews) |
SDS | |
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PBS.pdf | |
Glycerol.pdf | |
Proclin.pdf |