ETS1 associated protein II Positive Control peptide

Formulation:	Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Dilution Range:	WB: 1:1, 000
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	TDP2
Gene ID:	51567
Uniprot ID:	TYDP2_HUMAN

Tissue Specificity	Widely expressed. Highly expressed in various brain regions, including the frontal and occipital lobes, the hippocampus, the striatum and the cerebellum.
Post Translational Modifications	Ubiquitinated by TRAF6.
Function	DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Acts as a regulator of ribosome biogenesis following stress. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'-tyrosyl-DNA phosphodiesterase in cells. Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non-canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation. (Microbial infection) Also acts as a 5'-tyrosyl-RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA.
Peptide Name	Tyrosyl-Dna Phosphodiesterase 2 Tyr-Dna Phosphodiesterase 2 Htdp2 5'-Tyrosyl-Dna Phosphodiesterase 5'-Tyr-Dna Phosphodiesterase Ets1-Associated Protein 2 Ets1-Associated Protein Ii Eapii Traf And Tnf Receptor-Associated Protein Tyrosyl-Rna Phosphodiesterase Vpg Unlinkase
Database Links	Reactome: R-HSA-5693571
Cellular Localisation	Nucleus Pml Body Nucleolus Cytoplasm Localizes To Nucleolar Cavities Following Stress Localization To Nucleolus Is Dependent On Pml Protein (Microbial Infection) In Case Of Infection By Picornavirus Relocalizes To Cytoplasmic Sites Distinct From Those Containing Viral Proteins Associated With Rna Replication Or Encapsidation
Alternative Peptide Names	Tyrosyl-Dna Phosphodiesterase 2 protein Tyr-Dna Phosphodiesterase 2 protein Htdp2 protein 5'-Tyrosyl-Dna Phosphodiesterase protein 5'-Tyr-Dna Phosphodiesterase protein Ets1-Associated Protein 2 protein Ets1-Associated Protein Ii protein Eapii protein Traf And Tnf Receptor-Associated Protein protein Tyrosyl-Rna Phosphodiesterase protein Vpg Unlinkase protein TDP2 protein EAP2 protein TTRAP protein AD-022 protein

Information sourced from Uniprot.org

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