Biological Activity

Genistein is a highly specific inhibitor of protein tyrosine kinase (PTK) which blocks the mitogenic effect mediated by EGF on NIH-3T3 cells with IC50 of 12μM or by insulin with IC50 of 19 μM. Genistein inhibits protein tyrosine kinases including epidermal growth factor receptor kinase. Genistein also binds to PPARγ and estrogen receptors and acts as an agonist at GPR30.

Product Citations

• 

  Biomaterials. 2021 Aug;275:120958.

  Tumor progress intercept by intervening in Caveolin-1 related intercellular communication via ROS-sensitive c-Myc targeting therapy
  Genistein purchased from AbMole

Chemical Information

Molecular Weight	270.24
Formula	C15H10O5
CAS Number	446-72-0
Solubility (25°C)	DMSO 54 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Linda Yu, et al. Genistein: Dual Role in Women's Health

[2] Prakash Thangavel, et al. Genistein as Potential Therapeutic Candidate for Menopausal Symptoms and Other Related Diseases

[3] Neha Jaiswal, et al. An Overview on Genistein and its Various Formulations

[4] Vidya Mukund, et al. Genistein: Its role in metabolic diseases and cancer

[5] Carmela Spagnuolo, et al. Genistein and cancer: current status, challenges, and future directions