Application
| WB, IHC, ICC |
---|---|
Primary Accession | P14136 |
Other Accession | NP_001124491.1 |
Host | Mouse |
Isotype | IgG1 |
Reactivity | Human, Mouse, Rat |
Clonality | Monoclonal |
Description | Mouse Anti-Human GFAP R416WT Monoclonal IgG1 |
Target/Specificity | Detects ~50kDa. Does not cross-react with GFAP-R416W or other proteins (based on KO validation results). |
Other Names | Glial fibrillary acidic protein Antibody, Intermediate filament protein Antibody, Astrocyte Antibody, gfapl Antibody, DKFZp459C0729 Antibody, MGC139638 Antibody, FLJ45472 Antibody, AI836096 Antibody, GFAP Antibody |
Clone Names | S206B-9 |
Immunogen | Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse. >50% identity with other proteins (Vimentin, Desmin and Peripherin). |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | PBS pH 7.4, 50% glycerol, 0.1% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | 1 µg/ml of SMC-442 was sufficient for detection of GFAP R416WT in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Cellular Localization | Cytoplasm |
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Provided below are standard protocols that you may find useful for product applications.
Background
The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia.
References
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12. Thilenius, A.R.B., et al; J. Immunol. 162(2): 643-650 (1999).