K2143-100 assays | Glycogen Colorimetric/Fluorometric Assay Kit Clinisciences

Background

Glycogen is a multibranched polysaccharide of glucose in α-1,4 linkage with branching via α-1,6 linkage and serves as a form of energy storage in animals. Glycogen is the main storage form of glucose in body and is mainly synthesized in the liver and muscle. Abnormal ability to utilize glycogen is found in several genetic glycogen storage diseases and diabetes.

The Glycogen Colorimetric/Fluorometric Assay Kit provides a sensitive, fast and convenient way for accurate detection of glycogen levels in various biological samples based on colorimetric and fluorometric method. In the assay, glucoamylase hydrolyzes glycogen to glucose, which is subsequent specifically oxidized to generate a product that reacts with OxiRed probe to yield fluorescence (Ex 535/Em 587) and color (570 nm). The kit can detect 0.0004 to 2 mg/mL glycogen.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. 40 minutes; Kit contains all necessary reagents for accurate measurement of glycogen.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

Hydrolysis Buffer, Development Buffer, OxiRed Probe, Hydrolysis Enzyme Mix, Development Enzyme Mix, Glycogen Standard (2.0 mg/mL)

Glycogen Colorimetric/Fluorometric Assay Kit

Référence K2143-100 assays

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Marque : APExBIO Technology