Goat anti-ARIH1 / HHARI
Référence EB05812
Conditionnement : 100ug
Marque : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB05812 | Goat Anti-ARIH1 / HHARI Antibody | Pep-ELISA, WB, IHC | 100µg specific antibody in 200µl | Human |
Target Protein
Principal Names: ARIH1, HHARI, ARI, HARI, UBCH7BP, ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila), ariadne homolog, ariadne, Drosophila, homolog of, DKFZp686O13120, FLJ20329, FLJ93118, ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1Official Symbol: ARIH1
Accession Number(s): NP_005735.2
Human GeneID(s): 25820
Non-Human GeneID(s): 23806 (mouse)
Immunogen
Peptide with sequence C-HEGYEKDLWEYIED, from the C Terminus of the protein sequence according to NP_005735.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 65kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735]. Recommended for use at 0.2-1µg/ml. Primary incubated for 1 hour.
IHC: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Cow
Specific References
This antibody has been successfully used in IHC on Mouse:
Xiaolan Liu, Xufeng Cen, Ronghai Wu, Ziyan Chen, Yanqi Xie, Fengqi Wang, Bing Shan, Linghui Zeng, J Zhou, Bojian Xie, Yangjun Cai, Jin-Yan Huang, Yingjiqiong Liang, Youqian Wu, Chao Zhang, Dongrui Wang, Hong-Guang Xia
ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade.
Nat Commun. 2023 Jul 10;14(1):4066.
PMID: 37429863
This antibody has been successfully used in the following paper:
Youqian Wu, Chao Zhang, Xiaolan Liu, Zhengfu He, Bing Shan, Qingxin Zeng, Qingwei Zhao, Huaying Zhu, Hongwei Liao, Xufeng Cen, Xiaoyan Xu, Mengmeng Zhang, Tingjun Hou, Zhe Wang, Huanhuan Yan, Shuying Yang, Yaqin Sun, Yanying Chen, Ronghai Wu, Tingxue Xie, Wei Chen, Ayaz Najafov, Songmin Ying, and Hongguang Xia
ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation.
Nat Commun. 2021; 12: 2346.
PMID: 33879767
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol