• Specifications

    Product Description

    Goat anti-mouse IgG recognizes mouse IgG F(ab')2 fragment. This secondary antibody was purified using immunoaffinity chromatography. The antibody is conjugated with Fluorescein.

    Immunogen

    Mouse IgG F(ab')2 fragment

    Host

    Goat

    Reactivity

    Mouse

    Form

    Lyophilized

    Conjugation

    FITC

    Purification

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads.

    Conjugation Note

    3.0 moles Fluorescein (FITC) per mole of IgG

    Recommend Usage

    FLISA (1:10000-1:50000)
    Flow Cytometry (1:500-1:2500)
    Immunofluorescence (1:1000-1:5000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    Lyophilized from 0.01 M sodium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA, 0.01% sodium azide).

    Storage Instruction

    Store at 4°C on dry atmosphere.
    After reconstitution with 1 mL of deionized water (or equivalent), store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunofluorescence

    Flow Cytometry

  • Publication Reference