Goat F(ab')2 Anti-Mouse IgM, Human ads-PE

Référence 1022-09

Conditionnement : 0.25mg

Marque : Southern Biotech

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Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-PE
Specificity Reacts with the heavy chain of mouse IgM
Source Pepsin digest of Goat Anti-Mouse IgM, Human ads (SB Cat. No. 1020)
Cross Adsorption Mouse IgG and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Clonality Polyclonal
Concentration 0.25 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light; Do not freeze
Applications Quality tested applications for relevant formats include -
ELISA 1
FLISA
Flow Cytometry 2-7

Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 8
Immunohistochemistry-Paraffin Sections 9
Immunocytochemistry 10,11
Stimulation 12-25
Purification 26
RRID Number AB_2794265
Gene ID 16019 (Mouse)
Gene ID Symbol Ighm (Mouse)
Gene ID Aliases AI326478; Igh-6; Igh-M; Igh6; Igm; muH
UniProt ID P01872 (Mouse
UniProt Name IGHM_MOUSE (Mouse)
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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  2. 2. Yan J, Vetvicka V, Xia Y, Coxon A, Carroll MC, Mayadas TN, et al. β-glucan, a "specific" biologic response modifier that uses antibodies to target tumors for cytotoxic recognition by leukocyte complement receptor type 3 (CD11b/CD18). J Immunol. 1999;163:3045-52. (FC)
  3. 3. Stabel TJ, Bolin SR, Pesch BA, Rahner TE. A simple and rapid flow cytometric method for detection of porcine cell surface markers. J Immunol Methods. 2000;245:147-52. (FC)
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  7. 7. Guikema JE, Schrader CE, Leus NG, Ucher A, Linehan EK, Werling U, et al. Reassessment of the role of Mut S homolog 5 in Ig class switch recombination shows lack of involvement in cis- and trans-switching. J Immunol. 2008;181:8450-9. (FC)
  8. 8. Geraldes P, Rebrovich M, Herrmann K, Wong J, Jäck H, Wabl M, et al. Ig heavy chain promotes mature B cell survival in the absence of light chain. J Immunol. 2007;179:1659-68. (IHC-FS)
  9. 9. van Ginkel FW, Wahl SM, Kearney JF, Kweon M, Fujihashi K, Burrows PD, et al. Partial IgA-deficiency with increased Th2-type cytokines in TGF-β1 knockout mice. J Immunol. 1999;163:1951-7. (IHC-PS)
  10. 10. Montalvão F, Almeida GM, Silva EM, Borges VM, Vasconcellos R, Takiya CM, et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-α secretion and reduce parasite replication in macrophages. Eur J Immunol. 2010;40:417-25. (ICC)
  11. 11. Miyazaki A, Yogosawa S, Murakami A, Kitamura D. Identification of CMTM7 as a transmembrane linker of BLNK and the B-cell receptor. PLoS One. 2012;7(2):e31829. (ICC)
  12. 12. Takeshita H, Taniuchi I, Kato J, Watanabe T. Abrogation of autoimmune disease in Lyn-deficient mice by the mutation of the Btk gene. Int Immunol. 1998;10:435-44. (Stim)
  13. 13. Oliver AM, Martin F, Kearney JF. IgMhighCD21high lymphocytes enriched in the splenic marginal zone generate effector cells more rapidly than the bulk of follicular B cells. J Immunol. 1999;162:7198-207. (Stim)
  14. 14. Ochi H, Watanabe T. Negative regulation of B cell receptor-mediated signaling in B-1 cells through CD5 and Ly49 co-receptors via Lyn kinase activity. Int Immunol. 2000;12:1417-23. (Stim)
  15. 15. Kato I, Takai T, Kudo A. The pre-B cell receptor signaling for apoptosis is negatively regulated by FcγRIIB. J Immunol. 2002;168:629-34. (Stim)
  16. 16. Gavin AL, Aït-Azzouzene D, Ware CF, Nemazee D. ΔBAFF, an alternate splice isoform that regulates receptor binding and biopresentation of the B cell survival cytokine, BAFF. J Biol Chem. 2003;278:38220-8. (Stim)
  17. 17. Chattopadhyay G, Khan AQ, Sen G, Colino J, duBois W, Rubtsov A, et al. Transgenic expression of Bcl-xL or Bcl-2 by murine B cells enhances the in vivo antipolysaccharide, but not antiprotein, response to intact Streptococcus pneumoniae. J Immunol. 2007;179:7523-34. (Stim)
  18. 18. Bahjat FR, Pine PR, Reitsma A, Cassafer G, Baluom M, Grillo S, et al. An orally bioavailable spleen tyrosine kinase inhibitor delays disease progression and prolongs survival in murine lupus. Arthritis Rheum. 2008;58:1433-44. (Stim)
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  20. 20. Woo A, Kim J, Jeong Y, Maeng HG, Lee Y, Kang JS, et al. Vitamin C acts indirectly to modulate isotype switching in mouse B cells. Anat Cell Biol. 2010;43:25-35. (Stim)
  21. 21. Hauser J, Grundström C, Grundström T. Allelic exclusion of IgH through inhibition of E2A in a VDJ recombination complex. J Immunol. 2014;192:2460-70. (Stim)
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Référence
Description
Cond.
Prix HT
1021-09
 0.5mg 
1021-09S
 0.25mg