H460cells
Référence C0016003
Conditionnement : OneFrozenvial
Disease:
Large cell lung carcinoma
Origin:
Derived from metastatic pleural effusion
Species:
Homo sapiens
Tissue:
Lung
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
This is a hypotriploid human cell line
Patient:
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Subculture:
1:3 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
ISterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Isoenzymes:
AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 1DNA Profile (STR):
Amelogenin: X,Y; CSF1PO: 11,12; D13S317: 13; D16S539: 9; D5S818: 9,10; D7S820: 9,12; THO1: 9.3; TPOX: 8; vWA: 17If use of this culture results in a scientific publication, it should be cited in the publication as: H460 (AddexBio C0016003)
Related cells by Organ:
A427 cells
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H1650 cells
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H1975 cells
H226 cells
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H460 cells
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SW1573 cells
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References for H460 cells:
1. Lim, S. J., Choi, M. K., Kim, M. J., and Kim, J. K. Alpha-tocopheryl succinate potentiates the paclitaxel-induced apoptosis through enforced caspase 8 activation in human H460 lung cancer cells. Exp Mol Med, 41: 737-745, 2009.
2. Pesic, M., Podolski, A., Rakic, L., and Ruzdijic, S. Purine analogs sensitize the multidrug resistant cell line (NCI-H460/R) to doxorubicin and stimulate the cell growth inhibitory effect of verapamil. Invest New Drugs, 28: 482-492.
3. Liu, H., Li, L., Li, X. Q., Liu, X. J., and Zhen, Y. S. Enediyne lidamycin enhances the effect of epidermal growth factor receptor tyrosine kinase inhibitor, gefitinib, in epidermoid carcinoma A431 cells and lung carcinoma H460 cells. Anticancer Drugs, 20: 41-49, 2009.
4. Mollah, M. L., Song, J. C., Park, C. H., Lee, G. D., Hong, J. H., Ryoo, Z. Y., Lee, S., Chang, K. T., and Kim, K. S. Anticancer activity and apoptotic effects of Bulnesia sarmienti against human lung cancer H460 cells. Oncol Res, 18: 259-267, 2009.
5. Borgovan, T., Bellistri, J. P., Slack, K. N., Kopelovich, L., Desai, M., and Joe, A. K. Inhibition of BCL2 expression and activity increases H460 sensitivity to the growth inhibitory effects of polyphenon E. J Exp Ther Oncol, 8: 129-144, 2009.
6. Pesic, M., Andjelkovic, T., Bankovic, J., Markovic, I. D., Rakic, L., and Ruzdijic, S. Sulfinosine enhances doxorubicin efficacy through synergism and by reversing multidrug resistance in the human non-small cell lung carcinoma cell line (NCI-H460/R). Invest New Drugs, 27: 99-110, 2009.