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> Hamster Anti-Mouse TCRβ-AF647

Hamster Anti-Mouse TCRβ-AF647

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Référence 1785-31

Conditionnement : 0.1mg

Marque : Southern Biotech

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Product Specifications

More Information
Clone H57-597
Isotype Hamster (Armenian) IgG2
Isotype Control Hamster IgG-AF647
Specificity Mouse TCRβ
Description The αβ TCR is expressed on T lymphocytes of all mouse strains tested. The H57-597 monoclonal antibody does not react with γδ TCR-bearing cells. Plate-bound or soluble H57-597 activates αβ TCR-bearing T cells. In vitro and in vivo, H57-597 can induce immature thymocytes to undergo apoptosis. In vivo administration of H57-597 has been shown to deplete αβ TCR-bearing cells to near completion and prevent graft rejection.
Immunogen TCR affinity purified from mouse T-cell hybridoma DO-11.10
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.2 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 1,8-12
Immunohistochemistry –Reported in literature 7
Immunocytochemistry – Reported in literature 6
Immunoprecipitation – Reported in literature 1
Stimulation – Reported in literature 1,2
Depletion – Reported in literature 3,4
Separation – Reported in literature 5

RRID Number AB_2795290
Gene ID 21577 (Mouse)
Gene ID Symbol Tcrb (Mouse)
Gene ID Aliases TCRbeta; Tib

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

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Other Conjugates and Formulations

UNLB BIOT PE APC SPRD LE/AF PE/CY5.5 PACBLU AF700 AF488 FITC

Citations

  1. 1. Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine αβ T cell receptors. J Immunol. 1989;142:2736-42. (Immunogen, FC, IP, Stim)
  2. 2. Zerrahn K, Held W, Raulet DH. The MHC reactivity of the T cell repertoire prior to positive and negative selection. Cell. 1997;88:627-36. (Stim)
  3. 3. Davenport C, Kumar V, Bennett M. Rapid rejection of H2k and H2k/b bone marrow cell grafts by CD8+ T cells and NK cells in irradiated mice. J Immunol. 1995;155:3742-9. (Depletion)
  4. 4. Atsuta N, Nishimura H, Nakamura N, Emoto M, Iwatsuki T, Yoshokai Y. Diversity of Vγ gene segments rearranged to the Jγ4 gene in mice. J Immunol. 1995;154:676-84. (Depletion)
  5. 5. Lagrota-Candido J, Vasconcellos R, Cavalcanti M, Bozza M, Savino W, Quirico-Santos T. Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon-γ production. Int J Exp Path. 2002;83:121-32. (Sep)
  6. 6. Dong L, Watanabe K, Itoh M, Huan C, Tong X, Nakamura T, et al. CD4+ T-cell dysfunctions through the impaired lipid rafts ameliorate concanavalin A-induced hepatitis in sphingomyelin synthase 1-knockout mice. Int Immunol. 2012;24:327-37. (ICC)
  7. 7. Chatenoud L, Thervet E, Primo J, Bach J. Anti-CD3 antibody induces long-term remission of overt autoimmunity in nonobese diabetic mice. Proc Natl Acad Sci USA. 1994;91:123-7. (IHC)
  8. 8. Buslepp J, Kerry SE, Loftus D, Frelinger JA, Appella E, Collins EJ. High affinity xenoreactive TCR:MHC interaction recruits CD8 in absence of binding to MHC. J Immunol. 2003;170:373-83. (FC)
  9. 9. Galkina E, Tanousis K, Preece G, Tolaini M, Kioussis D, Florey O, et al. L-selectin shedding does not regulate constitutive T cell trafficking but controls the migration pathways of antigen-activated T lymphocytes. J Exp Med. 198;9:1323-35. (FC)
  10. 10. Rodríguez-Borlado L, Barber DF, Hernández C, Rodríguez-Marcos MA, Sánchez S, Hirsch E, et al. Phosphatidylinositol 3-kinase regulates the CD4/CD8 T cell differentiation ratio. J Immunol. 2003;170:4475-82. (FC)
  11. 11. Woolf E, Xiao C, Fainaru O, Lotem J, Rosen D, Negreanu V, et al. Runx3 and Runx1 are required for CD8 T cell development during thymopoiesis. Proc Natl Acad Sci USA. 2003;100:7731-6. (FC)
  12. 12. Conrad ML, Davis WC, Koop BF. TCR and CD3 antibody cross-reactivity in 44 species. Cytometry. 2007;71A:925-33. (FC, Porcupine Reactivity)