Hippeastrum hybrid (HHL)

Affinity purified Hippeastrum hybrid lectin (HHL) is consists of four subunits of identical size. It binds only to (alpha-1,3) and (alpha-1,6) linked mannosyl units of alpha-mannose residues. HHL reacts not only with terminal but also with internal alpha-D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1,6-linked alpha-mannosyl. HHL also binds to some yeast galactomannans.||Carbohydrate Specificity:|alpha-Mannose||Inhibitory Carbohydrate:|alpha(1,3) or alpha(1,6) linked mannosyl units||Divalent Ions:|None Required||Activity:|Agglutinates rabbit but not human erythrocytes||Storage and Stability:|Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.