Product Description  

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper), including a new generation of reverse transcriptase HiScript III Reverse Transcriptase and Buffer optimized for reverse transcription, further improving the efficiency of 1st strand synthesis. The 5 × gDNA wiper Mix in the kit can quickly remove genomic DNA contamination within 2 min, which makes the results more reliable and simplifies the process of qPCR primer design without the need of spanning an exon-exon junction. The kit contains single-component reverse transcription primers Oligo (dT)₂₀VN and Random hexamers, which allows users to choose primers flexibly for subsequent experiments as required. The kit can be used to synthesize full-length cDNA (up to 20 kb)  for cloning and other downstream experiments, as well as highly uniform cDNA for qPCR.

 Features  

• Excellent reverse transcription efficiency: Compatible with low RNA input and degraded RNA template.

• Superior reverse transcription performance: The length of obtained cDNA can reach to 20 kb.
  

• High impurities tolerance: It maintains efficient reverse transcription performance in the presence of common impurities (e.g. ethanol, isopropyl alcohol, water balance phenol).

• Selectable primers: Oligo (dT)₂₀VN and Random hexamers are available individually packaged.

• Efficiency gDNA elimination: Effective elimination of up to 500 ng of gDNA.

 Components  

 Storage  

Store at -30 ~ -15°C and transport at ≤0°C.