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> Histone H3K4me3 (Tri-methyl Lys4) antibody

Histone H3K4me3 (Tri-methyl Lys4) antibody

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Référence GTX128954-100ul

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Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in ChIP assay (ChIP assay). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Dot blot (Dot). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Whole mount) (IHC-Wm). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, Dot, ChIP assay, EM

Reactivity

 Human, Mouse, Rat, Zebrafish, Drosophila
Package
100 μl,
25 μl
Datasheet File
View product citations for antibody GTX128954 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Dot Assay dependent
ChIP assay Assay dependent
EM Assay dependent
Not tested in other applications.

Positive Control

whole zebrafish

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys4 of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2783841

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954

GTX128954 IHC-P Image

Histone H3K4me3 (Tri-methyl Lys4) antibody detects Histone H3K4me3 (Tri-methyl Lys4) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K4me3 (Tri-methyl Lys4) stained by Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in ChIP assay (ChIP assay). GTX128954

GTX128954 ChIP assay Image

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-Histone H3K4me3 (trimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GADPH gene.

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954

GTX128954 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954

GTX128954 WB Image

Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Dot blot (Dot). GTX128954

GTX128954 Dot Image

Dotblot analysis of anti-Histone H3 (tri-Methyl Lys4) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (tri-Methyl Lys4) antibody (GTX128954) 1:5000 dilution.
A: Peptide samples of H3K4 un
B: Peptide samples of H3K4me1
C: Peptide samples of H3K4me2
D: Peptide samples of H3K4me3
E: Peptide samples of H3K4ac
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Whole mount) (IHC-Wm). GTX128954

GTX128954 IHC-Wm Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:100.

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128954

GTX128954 ICC/IF Image

Histone H3 (tri-Methyl Lys4) antibody detects Histone H3 (tri-Methyl Lys4) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 (tri-Methyl Lys4) protein stained by Histone H3 (tri-Methyl Lys4) antibody (GTX128954) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954

GTX128954 IHC-P Image

Histone H3K4me3 (Tri-methyl Lys4) antibody detects Histone H3K4me3 (Tri-methyl Lys4) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Histone H3K4me3 (Tri-methyl Lys4) stained by Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128954

GTX128954 ICC/IF Image

Histone H3K4me3 (Tri-methyl Lys4) antibody detects Histone H3K4me3 (Tri-methyl Lys4) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K4me3 (Tri-methyl Lys4) stained by Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954

GTX128954 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Application Reference

Z Wang et al. Cell Rep 2024; 43 (6) : 114324 Tissue-resident trained immunity in hepatocytes protects against septic liver injury in zebrafish
JL Liu et al. Cell Metab 2024; 36 (6) : 1320-1334.e9 Dysfunctional circadian clock accelerates cancer metastasis by intestinal microbiota triggering accumulation of myeloid-derived suppressor cells
Application : WB
Reactivity : Mouse
Shangguan H et al. G3 (Bethesda) 2024; 14 (3) Knockdown of Kmt2d leads to growth impairment by activating the Akt/β-catenin signaling pathway.
Pang L et al. Biosci Rep 2021; 41 (11) KMT2D deficiency disturbs the proliferation and cell cycle activity of dental epithelial cell line (LS8) partially via Wnt signaling.
Application : IHC-Fr
Reactivity : Mouse
Chiu YF et al. Sci Rep 2020; 10 (1) : 8261 Critical role of SOX2-IGF2 signaling in aggressiveness of bladder cancer.
Submit a Reference

REVIEW

Histone H3K4me3 (Tri-methyl Lys4) antibody Cat. No. GTX128954

Rating
( Average 4 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Electron microscopy(EM)
( Average 3 based on 1 users reviews)
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
Lane Description : Lane 1: MG63 whole cell lysate, Lane 2: doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Electron microscopy (EM). GTX128954
Date : Anonymous submitted on 27-Mar-2017
Score :
Application Tested : EM
Sample Species : Ms
Sample : liver tissue
Blocking : Normal Goat Serum in PBS, 25°C, 0.08Hr
Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2)
Permeabilization /Antigen retrieval : 0.1% BSA in PBST
Primary Antibody : 1:10, 4°C, 16Hr
Application Reference
Z Wang et al. Cell Rep 2024; 43 (6):114324 Tissue-resident trained immunity in hepatocytes protects against septic liver injury in zebrafish
JL Liu et al. Cell Metab 2024; 36 (6):1320-1334.e9 Dysfunctional circadian clock accelerates cancer metastasis by intestinal microbiota triggering accumulation of myeloid-derived suppressor cells
Application : WB
Reactivity : Mouse
Shangguan H et al. G3 (Bethesda) 2024; 14 (3) Knockdown of Kmt2d leads to growth impairment by activating the Akt/β-catenin signaling pathway.
Pang L et al. Biosci Rep 2021; 41 (11) KMT2D deficiency disturbs the proliferation and cell cycle activity of dental epithelial cell line (LS8) partially via Wnt signaling.
Application : IHC-Fr
Reactivity : Mouse
Chiu YF et al. Sci Rep 2020; 10 (1):8261 Critical role of SOX2-IGF2 signaling in aggressiveness of bladder cancer.
Di Giorgio E et al. Nucleic Acids Res 2020; 48 (2):646-664 Different class IIa HDACs repressive complexes regulate specific epigenetic responses related to cell survival in leiomyosarcoma cells.
Application : WB
Laurent C et al. Blood 2019; (Epub) Gene alterations in epigenetic modifiers and JAK-STAT signaling are frequent in breast implant-associated ALCL.
Meng M et al. Immunogenetics 2019; 71 (7):489-499 Methylation of H3K27 and H3K4 in key gene promoter regions of thymus in RA mice is involved in the abnormal development and differentiation of iNKT cells.
Di Giorgio E et al. PLoS Genet 2017; 13 (4):e1006752 The co-existence of transcriptional activator and transcriptional repressor MEF2 complexes influences tumor aggressiveness.
Application : ChIP assay
Reactivity : Human
Eid W et al. Biol Chem 2016; (Epub) Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

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