A3466-100mg | Hoechst 33258 [23491-45-4] Clinisciences

Description: IC50 Value: N/A Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C. The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm. in vitro: N/A in vivo: N/A Clinical trial: N/A

1. Yi-fu He, Xi-min Hu, et al. "HSF1 Alleviates Brain Injury by Inhibiting NLRP3-Induced Pyroptosis in a Sepsis Model." Mediators of Inflammation. 27 Jan 2023.

Storage	Store at -20°C
M.Wt	533.88
Cas No.	23491-45-4
Formula	C₂₅H₂₇Cl₃N₆O
Synonyms	bisBenzimide H 33258;H 33258;Hoechst33258;Hoechst-33258
Solubility	≥42.5 mg/mL in H2O with gentle warming; insoluble in EtOH; ≥46.7 mg/mL in DMSO with gentle warming
Chemical Name	4-(6-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo23imidazol]-2'-yl)phenol trihydrochloride
SDF	Download SDF
Canonical SMILES	CN(CC1)CCN1C(C=C2N3)=CC=C2N=C3C4=CC=C5C(NC(C6=CC=C(O)C=C6)=N5)=C4.Cl.Cl.Cl
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Hoechst 33258 [23491-45-4]

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