Human CD11b/Mac-1a Antibody : APC
Référence OASB01039
Conditionnement : 100tests
Marque : Aviva Systems Biology
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Human CD11b/Mac-1a Antibody : APC (OASB01039)
| Datasheets/Manuals | Printable datasheet for Human CD11b/Mac-1a Antibody : APC (OASB01039) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | ICRF44 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | APC |
| Application | FC |
| Additional Information | Description: CD11b is the 165 kDa alphaM chain which combines with the CD18 beta2 integrin subunit to form the Mac-1 heterodimer. It is expressed on activated lymphocytes, monocytes, granulocytes and a subset of NK cells. CD11b functions in cell-cell and cell-substrate interactions and is a ligand for C3bi, CD50 (ICAM-3), CD54 (ICAM-1) and CD102 (ICAM-2). |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Rheumatoid synovial cells and human monocytes |
| Concentration | Lot specific |
| Specificity | CD11b |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform with thecharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified (UNLB) antibody Biotin conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate AF647 conjugate <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry, Blocking studies, Immunohistochemistry (frozen sections) |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.0. Store at 2- 8 C - The biotin (BIOT) and Alexa Fluor 647 (AF647) conjugates are supplied as 100 tests in 1.0 mL ofphosphate buffered saline containing 0.1% PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
| Reference | 1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, OxfordUniversity Press, Oxford 2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: WhiteCell Differentiation Antigens, Oxford University Press, Oxford 3. Springer, T.A. 1994. Cell 76:301 4. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD11b Section, Academic Press, New York, p. 158 5. Lee, J.O., L.A. Bankston, M.A. Arnaout, and R.C. Liddington. 1995. Structure 3:1333 6. Zhang, L.., and E.F. Plow. 1996. J. Biol. Chem. 271:18211 7. Davis, G.E. 1992. Exp. Cell. Res. 200:242 8. Perry, H.R., and R.F. Todd, III. 1996. Immunol. Today 17:209. |
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| Gene Symbol | ITGAM |
| Gene Full Name | Integrin alpha-M |
| Alias Symbols | CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6 |
| NCBI Gene Id | 3684; 397459; 714832; 101866035; 454069; 101004087 |
| Protein Name | integrin alpha-M |
| Description of Target | This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Uniprot ID | P11215, A0A1D5QVS1, A0A2K5UU26, H2QB02, A0A2J8J555 |
| Protein Accession # | NP_000623.2 |
| Nucleotide Accession # | NM_000632.3 |
