Human Cortisol ELISA kit

Référence EA100853

Conditionnement : 1x96wells

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Human Cortisol ELISA kit

SKU
EA100853
Human Cortisol ELISA kit
Specifications
Product Data
Assay Length 3 hours
Assay Platform 96-well plate
Assay Type Streptavidin based ELISA
Assay Type Sandwich
Background Cortisol (hydrocortisone, compound F) is the most potent glucocorticoid synthesized from cholesterol. Cortisol is found in the blood either as free Cortisol, or bound to corticosteroid-binding globulin (CBG). Cortisol production has an ACTH- dependent circadian rhythm with peak levels in the early morning and a nadir at night. The factors controlling this circadian rhythm are not completely defined. Serum levels are highest in the early morning and decrease throughout the day. In the metabolic aspect, Cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Immunologically, Cortisol functions as an important anti inflammatory, and plays a role in hypersensitivity, immunosuppression, and disease resistance. It has also been shown that plasma Cortisol levels elevate in response to stress. Abnormal Cortisol levels are seen with a variety of different conditions: with adrenal tumors, prostate cancer, depression, and schizophrenia. Elevated Cortisol levels and lack of diurnal variation have been identified in patients with Cushing's disease.
Components
  • 1. Streptavidin coated microwells: 12x8x1
  • 2. Cortisol Standard: 6 vials ( ready to use): 0.5 ml
  • 3. Biotin Reagent: 1 bottle (ready to use)
  • 4. Enzyme Conjugate (20X): 0.7 ml
  • 5. Assay Diluent: 1 bottle (ready to use): 12 ml
  • 6. TMB Substrate: 1 bottle (ready to use): 12 ml
  • 7. Stop Solution: 1 bottle (ready to use): 12 ml
  • 8. 20X Wash concentrate: 1 bottle: 25 ml
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Curve Range 20-400 ng/ml
Format 8x12 divisible strips
Interference No significant interference observed with available related molecules.
Reactivity Human
Reactivity Human
Reactivity Human
Sample Type Serum
Sample Volume 25 µl/well
Sensitivity 20 ng/ml
Signal Colorimetric
Species Human
Specificity This kit is used for quantitative detection human Cortisol
New No
Description Human Cortisol ELISA kit

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