Human NAAA (N-Acylethanolamine Acid Amidase) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	NAAA
Gene ID:	27163
Uniprot ID:	NAAA_HUMAN
Sample Type:	tissue homogenates, cell lysates or other biological fluids.

Tissue Specificity	Expressed in numerous tissues, with highest levels in liver and kidney, followed by pancreas.
Post Translational Modifications	N-glycosylated. Tunicamycin treatment causes a reduction in specific activity against N-palmitoylethanolamine. A disulfide bond is seen in the crystal structure of the human protein, but the Cys residues are not conserved in rodents. Autoproteolytic cleavage at pH 4.5 gives rise to the alpha and beta subunit. Cleavage gives rise to a conformation change that activates the enzyme. The same catalytic Cys residue mediates the autoproteolytic cleavage and subsequent hydrolysis of lipid substrates (Probable).
Function	Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine > N-arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine.
Protein Name	N-Acylethanolamine-Hydrolyzing Acid Amidase Acid Ceramidase-Like Protein Acylsphingosine Deacylase Naaa N-Acylsphingosine Amidohydrolase-Like Asah-Like Protein Cleaved Into - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Alpha - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Beta
Database Links	Reactome: R-HSA-112310
Cellular Localisation	Lysosome Membrane Peripheral Membrane Protein
Alternative ELISA Names	N-Acylethanolamine-Hydrolyzing Acid Amidase ELISA kit Acid Ceramidase-Like Protein ELISA kit Acylsphingosine Deacylase Naaa ELISA kit N-Acylsphingosine Amidohydrolase-Like ELISA kit Asah-Like Protein Cleaved Into - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Alpha - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Beta ELISA kit NAAA ELISA kit ASAHL ELISA kit PLT ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance