PC2017-10ml | HyperChrom CM HP Agarose Clinisciences

Description

HyperChrom CM HP Agarose is a weak cation exchange chromatography medium that separates molecules using differences in charge properties and amounts under specific conditions. It was found that when sodium hydroxide solution was continuously added dropwise to the chromatography medium, measuring the pH of the supernatant found that it could maintain a fairly wide range of pH stability. It is precisely because of this property that HyperChrom CM HP Agarose chromatography media exhibits an unexpected separation and purification effect. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.

HyperChrom CM HP Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.

HyperChrom CM HP Agarose chromatography media parameters

Name	Description
Chromatography media type	Weak cation exchange
Ligation	-O-CH₂COO^-
Scaffolding	Highly cross-linked agarose
Average particle size	34 μm
Ion load	0.06-0.08 mmol H+ /mL chromatography medium
Dynamic load	> 50 mg lysozyme/mL chromatography medium^*
Flow rates are recommended	90-150 cm/h
Maximum flow rate	200 cm/h
Withstand pressure	0.3 MPa
Use temperature	4-30℃
pH stability ^**	2-14
Solvent resistant	Common aqueous solution, 30% isopropanol^*, 75% ethanol^*, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea
Intolerant to solvents	Oxidant, cationic detergent
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.1 M citrate solution, pH 3.0; test sample: 4 mg/mL lysozyme sample, when the penetration of lysozyme reaches 10%, the lysozyme sample volume per unit volume (mL) (mg). After 7 days in the environment of chromatography at 40^oC and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. *30% and 75% are v/v, volume ratio.

Quality Control

Protocol

Components and Storage

Components	PC2017-25 mL	PC2017-100 mL
HyperChrom CM HP Agarose	25 mL	100 mL
Store the components at 4℃ for 5 years.

HyperChrom CM HP Agarose

Référence PC2017-10ml

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Description

Quality Control

Components and Storage