PC2012-100ml | HyperChrom DEAE FF Agarose Clinisciences

Description

HyperChrom DEAE FF Agarose is a weak anion exchange chromatography medium that separates molecules by taking advantage of differences in charge properties and how much they are under specific conditions. It was found that when sodium hydroxide solution was continuously added dropwise to the chromatography medium, measuring the pH of the supernatant found that it could maintain a fairly wide range of pH stability. It is precisely because of this characteristic that HyperChrom DEAE FF Agarose chromatography media exhibits its unexpected separation and purification effect. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.

HyperChrom DEAE FF Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.

HyperChrom DEAE FF Agarose chromatography media parameters

Name	Description
Chromatography media type	Weak anion exchange
Ligation	-O-CH₂CH₂–N ⁺ (C₂H₅)₃H
Scaffolding	Highly cross-linked agarose
Average particle size	90 μm
Ion load	0.11-0.16 mmolCl^-/mL chromatography medium
Dynamic load	> 90 mg ovalbumin/mL chromatography medium^*
Flow rates are recommended	300-600 cm/h
Maximum flow rate	700 cm/h
Withstand pressure	0.3 MPa
Use temperature	4-30℃
pH stability ^**	2-14
Solvent resistant	Common aqueous solution, 30% isopropanol^*, 75% ethanol^*, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea
Intolerant to solvents	Oxidant, anionic detergent
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.05 M Tris-HCl solution, pH 8.0; test sample: 6 mg/ml ovalbumin sample, ovalbumin loading volume per unit volume (mL) (mg) when the penetration of ovalbumin reaches 10%. After 7 days in the environment of chromatography at 40℃ and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. *30% and 75% are v/v, volume ratio.

Quality Control

Protocol

Components and Storage

Components	PC2012-25 mL	PC2012-100 mL
HyperChrom DEAE FF Agarose	25 mL	100 mL
Store the components at 4℃ for 5 years.

HyperChrom DEAE FF Agarose

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Description

Quality Control

Components and Storage