IgG2a NEGATIVE CONTROL Negative/Isotype Control
Référence OOSA10397
Conditionnement : 100Tests
Marque : Aviva Systems Biology
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IgG2a NEGATIVE CONTROL Negative/Isotype Control (OOSA10397)
| Datasheets/Manuals | Printable datasheet for IgG2a NEGATIVE CONTROL Negative/Isotype Control (OOSA10397) |
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| Predicted Species Reactivity | Negative Control |
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| Product Format | Liquid. Phosphate buffered saline |
| Isotype | IgG2a |
| Application | FC |
| Additional Information | Approx Protein Conc: IgG concentration 0.1 mg/ml Buffer solution: Phosphate buffered saline pH7.2 |
| :: | Fusion Partner: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. Immunogen: Activated rat T-helper cells. |
| :: | Label Protein Molar Ratio: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Preservative: 0.09% - Sodium Azide 1% - Bovine Serum Albumin |
| Reconstitution and Storage | -20°C |
| Concentration | 0.1 mg/ml |
| Specificity | This product is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue. This antibody may not be suitable for intracellular staining on some cell types. Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control. Test results have shown that This product is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues. |
| Predicted Homology Based on Immunogen Sequence | Negative Control |
| Immunogen | Activated rat T-helper cells. |
| Purification | Protein A Purified |
| Reference | 1. Avigdor, A. et al. (2004) CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow. Blood. 103: 2981-2989. |
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| Description of Target | MOUSE IgG2a NEGATIVE CONTROL |