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> IL10 ELISA Kit (Guinea pig )

IL10 ELISA Kit (Guinea pig )

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Référence OKCD01447

Conditionnement : 96Wells

Marque : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for IL10 ELISA Kit (Guinea pig) (OKCD01447)
Product Info
Predicted Species ReactivityCavia porcellus|Guinea Pig
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)96-104%89-96%78-104%86-102%
EDTA Plasma (n=5)79-96%92-105%98-105%86-93%
Heparin Plasma (n=5)92-101%84-99%87-102%80-92%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 (IL10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 (IL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 10 (IL10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)94-10198
EDTA Plasma (n=5)82-10190
Heparin Plasma (n=5)78-9080
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IL10 Microplate96 Wells (12 x 8 Well Strips)
IL10 Lyophilized Standard2 x 200 pg
100X Biotinylated IL10 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 1.22 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 10 (IL10). No significant cross-reactivity or interference between Interleukin 10 (IL10) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIL10
Alias SymbolsCytokine synthesis inhibitory factor.
NCBI Gene Id100715618
Protein NameInterleukin-10
Description of TargetInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Uniprot IDQ9Z1Y5
Protein Accession #NP_001247414.1
Nucleotide Accession #NM_001260485.1
Protein Size (# AA)178