IL5 recombinant monoclonal antibody, clone 2E3

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

Flow cytometric analysis of human peripheral blood leukocytes (PBL) with IL5 recombinant monoclonal antibody, clone 2E3 (Cat # RAB03587).
Human PBL cells were fixed using 2% PFA and permeabilized with 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control-black line) or the r version of RAB03587 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Neutralization

Entrez GeneID

3567

Gene Name

IL5

Gene Alias

EDF, IL-5, TRF

Gene Description

interleukin 5 (colony-stimulating factor, eosinophil)

Omim ID

147850

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq

Other Designations

B cell differentiation factor I|OTTHUMP00000065830|T-cell replacing factor|eosinophil differentiation factor|interleukin 5|interleukin-5