Invertase antibody (Biotin)
Référence orb344292-100ug
Conditionnement : 100ug
Marque : Biorbyt
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Invertase antibody (Biotin)
Catalog Number: orb344292
| Catalog Number | orb344292 |
|---|---|
| Category | Antibodies |
| Description | Invertase antibody (Biotin) |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Yeast |
| Isotype | IgG |
| Immunogen | Invertase [Candida species] |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500 |
| Form/Appearance | Lyophilized |
| Purity | Anti-Invertase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Invertase [Candida species]. Cross reactivity against Invertase from other sources is unknown. |
| Conjugation | Biotin |
| UniProt ID | P10594 |
| NCBI | P40912.1 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | goat anti-Invertase Antibody biotin Conjugated, Bi |
| Note | For research use only |
| Application notes | Anti-Invertase antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Invertase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
Biorbyt Goat anti Invertase antibody (orb750394) was used to detect purified invertase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/orb348637 1 hr RT).