Jurkat E6.1 growing culture
Référence 330223
Conditionnement : 1cellcultureflask
Marque : CLS Cell Lines Service
Jurkat E6.1 Cells
Overview of Jurkat E6.1 cells
Description | Jurkat E6.1 cells, a derivative clone of the Jurkat cell line, which originates from the peripheral blood of a 14-year-old boy with acute T-cell leukemia, are a pivotal resource in the field of tumor immunology and leukemia research. These cells exhibit rapid proliferation and a pronounced responsiveness to stimuli, crucial for studying T cell biology, including T cell receptor (TCR) signaling, activation, proliferation, and apoptosis. Characterized by mutations such as the TEL-JAK2 fusion gene, Jurkat E6.1 cells provide insights into the leukemia phenotype and the molecular mechanisms underlying T-cell leukemia. Jurkat E6.1 cells are commonly used to investigate the intracellular signaling pathways that are activated upon TCR engagement, such as the NF-κB pathway, MAPK pathways, and calcium signaling, which are crucial for T cell activation and function. The cell line's responsiveness to phorbol esters and agents targeting the T3 antigen makes it an invaluable tool for exploring the intricacies of T-cell activation, including the induction of Interleukin-2 (IL-2) production. This feature, combined with their abnormal karyotype, underscores the utility of Jurkat E6.1 cells in research focused on the immune synapse architecture and the signaling pathways that govern T-cell proliferation and function. Jurkat E6.1 cells' utility extends to the study of apoptosis, offering a model to investigate the effects of various compounds, including alkaloids extract from sources such as Tribulus terrestris, on cell death pathways. This aspect is particularly relevant for identifying potential therapeutic agents and understanding their mechanisms of action in T-cell leukemia. In summary, Jurkat E6.1 cells, with their unique characteristics and versatility, continue to be a cornerstone in the study of T-cell activation, signaling, and apoptosis. |
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Organism | Human |
Tissue | Blood |
Disease | Acute T cell leukemia |
Metastatic site | T lymphocyte |
Synonyms | JurkatE6-1, Jurkat E6-1, Jurkat, Clone E6-1, Jurkat Clone E6-1, Jurkat (clone E6-1), JURKAT E-6.1, JURKAT E-61, Jurkat-E6, Jurkat E6, J.E6-1, E6-1 |
Properties
Age | 14 years |
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Gender | Male |
Morphology | Round cells |
Cell type | Lymphoblast |
Growth properties | Suspension |
Specifications of the Jurkat clone E6 cell line
Citation | Jurkat E6.1 (Cytion catalog number 300223) |
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Biosafety level | 1 |
Genetic profile
Antigen expression | CD3 |
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Products | Interleukin-2 (interleukin 2, IL-2), interferon gamma |
Karyotype | Modal number = 46, range = 41 to 47, the karyotype is 46,xY,-2,-18, del(2)(p21p23), del(18)(p11.2) |
Handling Jurkat E6.1 cells
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Seeding density | 1 x 10^5 cells/ml |
Fluid renewal | Every 2 days |
Freezing recovery | Fast |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,y CSF1PO: 11,12 D13S317: 8,12 D16S539: 11 D5S818: 9 D7S820: 8,10 TH01: 6,9.3 TPOX: 8,10 vWA: 18 D3S1358: 15 D21S11: 31.2,33.2 D18S51: 13,21 Penta E: 10,12 Penta D: 11,13 D8S1179: 13,14 FGA: 20,21 |
HLA alleles | A*: 03:01:01 B*: 07:02:01, 11:03:01 C*: 04:01:01, 07:02:01 DRB1*: 07:01:01, 15:01:01 DQA1*: 01:02:01, 02:01:01 DQB1*: 02:02:01, 06:03:01 DPB1*: 02:01:02G, 04:02:01G |